Overview

  • Product nameAnti-LSG1 antibody
    See all LSG1 primary antibodies
  • Description
    Rabbit polyclonal to LSG1
  • Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment: SELNDGYDWG RLNLQSVTEQ SSLDDFLATA ELAGTEFVAE KLNIKFVPAE ARTGLLSFEE SQRIKKLHEE NKQFLCIPRR PNWNQNTTPE E, corresponding to amino acids 37-127 of Human LSG1 (Q9H089).

  • Positive control
    • RT4, U-251 MG, human Liver and Human Tonsil lysates, Human cerebellum tissue, U-2 OS cells

Properties

Applications

Our Abpromise guarantee covers the use of ab122409 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.
ICC/IF Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100

Target

  • FunctionGTPase required for the XPO1/CRM1-mediated nuclear export of the 60S ribosomal subunit. Probably acts by mediating the release of NMD3 from the 60S ribosomal subunit after export into the cytoplasm.
  • Sequence similaritiesBelongs to the MMR1/HSR1 GTP-binding protein family. LSG1 subfamily.
    Contains 1 G (guanine nucleotide-binding) domain.
  • DomainIn contrast to other GTP-binding proteins, this family is characterized by a circular permutation of the GTPase motifs described by a G4-G1-G3 pattern.
  • Cellular localizationCytoplasm. Endoplasmic reticulum. Nucleus > Cajal body. Shuttles between the Cajal bodies in the nucleus and the endoplasmic reticulum.
  • Information by UniProt
  • Database links
  • Alternative names
    • hLsg1 antibody
    • Large subunit GTPase 1 homolog antibody
    • LSG1 antibody
    • LSG1_HUMAN antibody

Anti-LSG1 antibody images

  • Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)
    Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
  • All lanes : Anti-LSG1 antibody (ab122409) at 1/250 dilution

    Lane 1 : RT4 cell lysate
    Lane 2 : U-251 MG cell lysate
    Lane 3 : Human Plasma lysate
    Lane 4 : Human Liver lysate
    Lane 5 : Human Tonsil lysate
    10-20% SDS PAGE
  • ab122409 at 1/200 dilution staining LSG1 in paraffin-embedded Human cerebellum tissue by Immunohistochemistry.
  • ab122409 at a concentratio of 4 µg/ml staining Human cell line U2 OS using immunofluorescence. Note: shows positivity in nucleus & cytoplasm.

References for Anti-LSG1 antibody (ab122409)

ab122409 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"