The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
1/250 - 1/500.
Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100
FunctionGTPase required for the XPO1/CRM1-mediated nuclear export of the 60S ribosomal subunit. Probably acts by mediating the release of NMD3 from the 60S ribosomal subunit after export into the cytoplasm.
Sequence similaritiesBelongs to the MMR1/HSR1 GTP-binding protein family. LSG1 subfamily. Contains 1 G (guanine nucleotide-binding) domain.
DomainIn contrast to other GTP-binding proteins, this family is characterized by a circular permutation of the GTPase motifs described by a G4-G1-G3 pattern.
Cellular localizationCytoplasm. Endoplasmic reticulum. Nucleus > Cajal body. Shuttles between the Cajal bodies in the nucleus and the endoplasmic reticulum.