The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 15 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Use a concentration of 4 - 8 µg/ml.
RelevanceSm like proteins were identified in a variety of organisms based on sequence homology with the Sm protein family. Sm like proteins contain the Sm sequence motif, which consists of 2 regions separated by a linker of variable length that folds as a loop. The Sm like proteins are thought to form a stable heteromer present in tri snRNP particles, which are important for pre mRNA splicing.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human heart tissue labelling LSM4 with ab86205 at 4.0-8.0µg/ml. Positive staining shown in cardiac cells. Magnification: 400X.
Western blot - LSM4 antibody (ab86205)
Anti-LSM4 antibody (ab86205) at 1 µg/ml (5% skim milk/ PBS buffer) + Jurkat cell lysate at 10 µg
Secondary HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 15 kDa Gel concentration: 10-20%
References for Anti-LSM4 antibody (ab86205)
has not yet been referenced specifically in any publications.
Publishing research using ab86205? Please let us know so that we can cite the reference in this datasheet.
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