Recombinant Anti-LSM5 antibody [EPR10690] (ab184568)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10690] to LSM5
- Suitable for: Flow Cyt (Intra), ICC/IF, WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-LSM5 antibody [EPR10690]
See all LSM5 primary antibodies -
Description
Rabbit monoclonal [EPR10690] to LSM5 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- T47-D lysate, Human astrocytoma or stomach tissue, T47-D cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR10690 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab184568 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/110.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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ICC/IF |
1/500.
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WB |
1/1000 - 1/10000. Predicted molecular weight: 10 kDa.
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Notes |
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Flow Cyt (Intra)
1/110. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ICC/IF
1/500. |
WB
1/1000 - 1/10000. Predicted molecular weight: 10 kDa. |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
Plays a role in U6 snRNP assembly and function. Binds to the 3' end of U6 snRNA, thereby facilitating U4/U6 duplex formation in vitro. -
Sequence similarities
Belongs to the snRNP Sm proteins family. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 23658 Human
- Entrez Gene: 100044729 Mouse
- Entrez Gene: 66373 Mouse
- Entrez Gene: 306222 Rat
- Omim: 607285 Human
- SwissProt: Q9Y4Y9 Human
- SwissProt: P62322 Mouse
- Unigene: 424908 Human
see all -
Alternative names
- 2010208O10Rik antibody
- 2310034K10Rik antibody
- FLJ12710 antibody
see all
Images
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Intracellular Flow Cytometry analysis of T47-Dcells using ab184568at a 1/110 dilution (red) or aRabbit monoclonal IgG (negative) (green). Goat anti rabbit IgG (FITC) secondary usedat a 1/150 dilution.
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Immunofluorescence analysis of 4% paraformaldehyde fixed T47-D cells labeling LSM5 with ab184568 at a 1/500 dilution. Goat anti rabbit IgG (Alexa Fluor®555) secondary used at a 1/200 diution. Conterstained with Dapi (right image).
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Immunohistochemical analysis of paraffin embedded Human stomach tissue labeling LSM5 with ab184568 a 1/100 dilution. Counterstained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin embedded Human astrocytoma tissue labeling LSM5 with ab184568 at a 1/100 dilution. Counterstained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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Anti-LSM5 antibody [EPR10690] (ab184568) at 1/10000 dilution + T47-D lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 10 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab184568 has not yet been referenced specifically in any publications.