This epitope affinity purified rabbit polyclonal antibody reacts predominantly with LSP1. By immunoblot analysis, a ~52 kDa immunoreactive protein from Mouse Spleen tissue lysate was detected (sample were boiled prior to subject 10% SDSpolyacrylamide gel electrophoresis). This band is abolished by the depletion of the synthetic immunizing peptide.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.1 - 1 µg/ml.
Use 3µg for 105 cells.
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 52 kDa.
May play a role in mediating neutrophil activation and chemotaxis.
Phosphorylated by casein kinase II, protein kinase C and MAPKAPK2. Phosphorylation by PKC induces translocation from membrane to cytoplasm. Phosphorylation by MAPKAPK2 may regulate neutrophil chemotaxis.