The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesELISA: Use at a concentration of 0.01 - 0.1 µg/ml.
IHC-P: Use at a concentration of 2 - 5 µg/ml.
IP: Use at a concentration of 2 - 5 µg/ml.
WB: Use at a concentration of 0.1 - 1.0 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionMay play a role in mediating neutrophil activation and chemotaxis.
Tissue specificityActivated T-lymphocytes.
Post-translational modificationsPhosphorylated by casein kinase II, protein kinase C and MAPKAPK2. Phosphorylation by PKC induces translocation from membrane to cytoplasm. Phosphorylation by MAPKAPK2 may regulate neutrophil chemotaxis.