Anti-LSR antibody (ab191180)
Key features and details
- Rabbit polyclonal to LSR
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-LSR antibody
See all LSR primary antibodies -
Description
Rabbit polyclonal to LSR -
Host species
Rabbit -
Specificity
ab191180 was affinity purified using an epitope specific to LSR immobilized on a solid support. -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee, Gorilla -
Immunogen
Synthetic peptide within Human LSR aa 450-550. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q86X29 -
Positive control
- WB: HeLa, 293T and Jurkat whole cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab191180 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP |
Use at 2-10 µg/mg of lysate.
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WB |
1/2000 - 1/10000. Predicted molecular weight: 71 kDa.
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Notes |
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IP
Use at 2-10 µg/mg of lysate. |
WB
1/2000 - 1/10000. Predicted molecular weight: 71 kDa. |
Target
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Function
Probable role in the clearance of triglyceride-rich lipoprotein from blood. Binds chylomicrons, LDL and VLDL in presence of free fatty acids and allows their subsequent uptake in the cells. -
Sequence similarities
Belongs to the immunoglobulin superfamily. LISCH7 family.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 468834 Chimpanzee
- Entrez Gene: 101139687 Gorilla
- Entrez Gene: 51599 Human
- SwissProt: Q86X29 Human
- Unigene: 466507 Human
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Alternative names
- ILDR3 antibody
- Immunoglobulin like domain containing receptor 3 antibody
- Lipolysis Stimulated Lipoprotein Receptor antibody
see all
Images
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Immunoprecipitation of 293T whole cell lysate using ab191180 (lane 1) at 6µg per reaction (0.5 or 1 mg/IP; 20% of IP loaded/lane). Immunoblot analysis of LSR utilised ab191180 at 0.4 µg/ml. The lysate in lane 2 is precipitated with an IgG control antibody. Detection utilised chemiluminescence with an exposure time of 30seconds.
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All lanes : Anti-LSR antibody (ab191180) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate prepared using RIPA lysis buffer.
Lane 2 : 293T whole cell lysate prepared using RIPA lysis buffer.
Lane 3 : Jurkat whole cell lysate prepared using RIPA lysis buffer.
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 71 kDa
Exposure time: 3 minutes
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab191180 has been referenced in 1 publication.
- Tang X et al. Potentiation of cancerous progression by LISCH7 via direct stimulation of TGFB1 transcription in triple-negative breast cancer. J Cell Biochem 121:4642-4653 (2020). PubMed: 32048750