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Anti-LT subunit (A + B) antibody
Rabbit polyclonal to LT subunit (A + B)
ab188541 reacts with LT and cholera toxin.
Full length native protein (purified) corresponding to Escherichia coli LT subunit (A + B). Initial immunization with LT toxoid and booster with LT toxin.
Database link: P06717
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Predicted molecular weight: 30 kDa.
Use at an assay dependent concentration.
SDS-PAGE - Anti-E. coli heat labile toxin A antibody (ab188541)
SDS-PAGE analysis stained with CBB:
Lane 1. culture medium of ETEC
Lane 2. crude extract of ETEC cells
Western blot - Anti-E. coli heat labile toxin A antibody (ab188541)
All lanes :
Anti-LT subunit (A + B) antibody (ab188541) at 1/2000 dilution
Lane 1 :
Lane 2 :
Crude extract of ETEC cells
Predicted band size :
* Indicates a non-specific protein band.
Immunoprecipitation - (ab188541)
Immunopprecipitation of LT from culture medium of ETEC by using ab188541. H chain and L chain indicate heavy chain and light chain of IgG, respectively.
This product has been referenced in:
et al. Heat-Labile Enterotoxin-Induced PERK-CHOP Pathway Activation Causes Intestinal Epithelial Cell Apoptosis. Front Cell Infect Microbiol 7:244 (2017).
Read more (PubMed: 28642847) »
et al. Electron Acceptors Induce Secretion of Enterotoxigenic Escherichia coli Heat-Labile Enterotoxin under Anaerobic Conditions through Promotion of GspD Assembly. Infect Immun 84:2748-57 (2016).
Read more (PubMed: 27430271) »
See all 2 Publications
for this product
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"