Overview

  • Product nameAnti-LTBP1 antibody
    See all LTBP1 primary antibodies
  • Description
    Rabbit polyclonal to LTBP1
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat
    Predicted to work with: Cow, Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1350 - 1450 of Human LTBP1.

    (Peptide available as ab87128.)

  • Positive control
    • This antibody gave a positive signal in the following tissue lysates: Rat Liver; Mouse Pancreas; Mouse Spleen. ]# This antibody gave a positive result in IHC in the following FFPE tissue: Mouse spleen.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab78294 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 187 kDa (predicted molecular weight: 187 kDa).
IHC-P Use a concentration of 5 µg/ml.

Target

  • FunctionMay be involved in the assembly, secretion and targeting of TGFB1 to sites at which it is stored and/or activated. May play critical roles in controlling and directing the activity of TGFB1. May have a structural role in the extra cellular matrix (ECM).
  • Tissue specificityIsoform Long is found in fibroblasts.
  • Sequence similaritiesBelongs to the LTBP family.
    Contains 18 EGF-like domains.
    Contains 4 TB (TGF-beta binding) domains.
  • DomainAssociates covalently with small latent TGF-beta complex via domain TB 3.
  • Post-translational
    modifications
    Contains hydroxylated asparagine residues.
    Isoform Short N-terminus is blocked.
    The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • 9430031G15Rik antibody
    • 9830146M04 antibody
    • Latent transforming growth factor beta binding protein 1 antibody
    • Latent-transforming growth factor beta-binding protein 1 antibody
    • Latent-transforming growth factor beta-binding protein, isoform 1L antibody
    • Latent-transforming growth factor beta-binding protein, isoform 1S antibody
    • LBT1S antibody
    • LTBP-1 antibody
    • Ltbp1 antibody
    • LTBP1_HUMAN antibody
    • MGC163161 antibody
    • TGF-beta1-BP-1 antibody
    • Tgfb antibody
    • Transforming growth factor beta-1-binding protein 1 antibody
    • Transforming growth factor beta-1-masking protein large subunit antibody
    see all

Anti-LTBP1 antibody images

  • All lanes : Anti-LTBP1 antibody (ab78294) at 1 µg/ml

    Lane 1 : Liver (Rat) Tissue Lysate
    Lane 2 : Pancreas (Mouse) Tissue Lysate
    Lane 3 : Spleen (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 187 kDa
    Observed band size : 187 kDa
    Based on the immunogen sequence, this antibody is predicted to react with all three isoforms of LTBP1. The band detected here might correspond to the long isoform, which is 187 kDa and is found in fibroblasts.
  • IHC image of LTBP1 staining in Mouse spleen formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab78294, 5µg/ml, for 15 mins at room temperature. A Goat anti-Rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • All lanes : Anti-LTBP1 antibody (ab78294) at 1 µg/ml

    Lane 1 : Liver (Rat) Tissue Lysate
    Lane 2 : Spleen (Mouse) Tissue Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 187 kDa
    Observed band size : 187 kDa
    Additional bands at : 171 kDa (possible cleavage fragment),55 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds

References for Anti-LTBP1 antibody (ab78294)

This product has been referenced in:
  • Maurya VK  et al. Transforming growth factor-beta 1 (TGF-B1) liberation from its latent complex during embryo implantation and its regulation by estradiol in mouse. Biol Reprod 89:84 (2013). WB ; Mouse . Read more (PubMed: 23926286) »
  • Squadrito ML  et al. miR-511-3p modulates genetic programs of tumor-associated macrophages. Cell Rep 1:141-54 (2012). WB ; Mouse . Read more (PubMed: 22832163) »

See all 2 Publications for this product

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