Anti-LTBR antibody (ab226377)
Key features and details
- Rabbit polyclonal to LTBR
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-LTBR antibody
See all LTBR primary antibodies -
Description
Rabbit polyclonal to LTBR -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee, Gorilla, Orangutan -
Immunogen
Synthetic peptide within Human LTBR aa 385-435. The exact sequence is proprietary.
Database link: P36941 -
Positive control
- WB: HeLa, HEK-293T and Jurkat whole cell lysates. IP: HeLa whole cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab226377 was affinity purified using an epitope specific to LTBR immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab226377 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP |
Use at 2-10 µg/mg of lysate.
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WB |
1/1000 - 1/5000. Predicted molecular weight: 47 kDa.
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Notes |
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IP
Use at 2-10 µg/mg of lysate. |
WB
1/1000 - 1/5000. Predicted molecular weight: 47 kDa. |
Target
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Function
Receptor for the heterotrimeric lymphotoxin containing LTA and LTB, and for TNFS14/LIGHT. Promotes apoptosis via TRAF3 and TRAF5. May play a role in the development of lymphoid organs. -
Sequence similarities
Contains 4 TNFR-Cys repeats. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 451778 Chimpanzee
- Entrez Gene: 101149920 Gorilla
- Entrez Gene: 4055 Human
- Entrez Gene: 100171849 Orangutan
- Omim: 600979 Human
- SwissProt: P36941 Human
- Unigene: 1116 Human
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Alternative names
- CD18 antibody
- D12S370 antibody
- LT beta R antibody
see all
Images
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All lanes : Anti-LTBR antibody (ab226377) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 47 kDa
Exposure time: 30 secondsLysates prepared using NETN lysis buffer.
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LTBR was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (prepared using NETN lysis buffer; 1 mg for IP, 20% of IP loaded) with ab226377 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226377 at 0.4 µg/ml.
Lane 1: ab226377 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab226377 has not yet been referenced specifically in any publications.