Overview

  • Product nameAnti-Lubricin antibody
    See all Lubricin primary antibodies
  • Description
    Rabbit polyclonal to Lubricin
  • Specificityab28484 recognises Lubricin.
  • Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Cow, Human, African Green Monkey
    Predicted to work with: Horse
  • Immunogen

    Synthetic peptide:

    CLPNIRKQPDGYDYYAFSKDQ

    , corresponding to amino acids 1356-1374 of Human Lubricin

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.05% Sodium Azide
    Constituents: PBS, 1mg/ml BSA
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab28484 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 µg/ml. Detects a band of approximately 280 kDa (predicted molecular weight: 156 kDa).
ICC/IF Use at an assay dependent concentration.
IHC-P Use a concentration of 4 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • RelevanceLubricin is a glycoprotein encoded by the PRG4 gene. It has been identified as a component of joint lubrication and synovial homeostasis by reducing friction in joints and preventing synovial cell overgrowth. It is a large, water soluble molecule that acts as a carrier for insoluble surface-active phospholipid (SAPL). Depletion of lubricin function has been associated with camptodactyly-arthropathy-coxa vara-pericarditis syndrome (CACP), an arthritic-like autosomal recessive disorder.
  • Cellular localizationSecreted
  • Database links
  • Alternative names
    • Articular superficial zone protein antibody
    • bG174L6.2 antibody
    • CACP antibody
    • Camptodactyly arthropathy coxa vara pericarditis syndrome gene antibody
    • FLJ32635 antibody
    • HAPO antibody
    • Jacobs camptodactyly arthropathy pericarditis syndrome gene antibody
    • JCAP antibody
    • Megakaryocyte stimulating factor antibody
    • MSF antibody
    • PRG 4 antibody
    • PRG4 antibody
    • Proteoglycan 4 antibody
    • Proteoglycan4 antibody
    • Superficial zone proteoglycan antibody
    • SZP antibody
    see all

Anti-Lubricin antibody images

  • Anti-Lubricin antibody (ab28484)

    Predicted band size : 156 kDa
    Observed band size : 280 kDa (why is the actual band size different from the predicted?)

    Recombinant Human Lubricin detected with ab28484.



  • Predicted band size : 156 kDa

    Samples from normal bovine (NL bovine) and human osteoarthritic synovial fluids (OA human). The lubricin was purified by CsCl gradient and DEAE sepharose chromatography prior electrophoresis. ab28484 was used at a concentration of 2μg/ml

    ab28484 works best with non-reduced samples as it looses a lot of its binding capapcity if the lubricin is reduced prior to SDS PAGE. Western blot analysis is difficult unless the protein sample is a recominant or purified sample.

    The wide band, higher than expected molecular weight seen on the blot, are consistent with the high carbohydrate content of the protein sample.

  • ab28484 (4µg/ml) staining lubricin in Human liver using an automated system. Using this protocol there is staining of the cytoplasm and nuclei in hepatic arteries and plates of hepatic cells.
    Sections were rehydrated and antigen retrieved with  buffers EDTA pH 9.0 . Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked  for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected  for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

  • ab28484 staining Lubricin in COS-1 cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed in paraformaldehyde and permeabilized in 0.25% Triton X-100 prior to blocking in 1% BSA for 30 minutes at 22°C. The primary antibody was diluted 1/250 and incubated with the sample for 16 hours at 4°C. The secondary antibody was Alexa Fluor® 568-conjugated goat anti-rabbit polyclonal, diluted 1/400.

    For permeabilization, 0.25% Triton X-100 was included in primary and secondary antibody incubation buffers.

    Cells were also stained with FITC-labeled Peanut Agglutinin. PNA binds lubricin and certain other glycoproteins, so some co-localization was expected between the Alexa Fluor 568 signal and the FITC signal.

    Unexpected co-localization was noted between the Alexa Fluor 568 staining (indirect lubricin staining) and DAPI (nuclear counterstain) in the cell nuclei.

    See Abreview

References for Anti-Lubricin antibody (ab28484)

This product has been referenced in:
  • Bhumiratana S  et al. Large, stratified, and mechanically functional human cartilage grown in vitro by mesenchymal condensation. Proc Natl Acad Sci U S A 111:6940-5 (2014). Read more (PubMed: 24778247) »
  • Ruan MZ  et al. Proteoglycan 4 expression protects against the development of osteoarthritis. Sci Transl Med 5:176ra34 (2013). IHC ; Mouse . Read more (PubMed: 23486780) »

See all 5 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (cartilage)
Antigen retrieval step None
Permeabilization No
Specification cartilage
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Fixative zinc buffered formalin
Username

Abcam user community

Verified customer

Submitted Feb 15 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (synovium)
Antigen retrieval step None
Permeabilization No
Specification synovium
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Fixative zinc buffered formalin
Username

Abcam user community

Verified customer

Submitted Feb 15 2016

Application Western blot
Sample Human Cell lysate - whole cell (neuroepithelioma cells)
Gel Running Conditions Reduced Denaturing
Loading amount 20 µg
Specification neuroepithelioma cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Aug 26 2015

DISCOUNT CODE: XXXXXXX
Expiration date: 05-06-2013
Value: €380

I am very pleased to hear you would like to accept our offer and test ab28484 in Prcine. This code will give you $380 off your next order before the expiration date. T...

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I am very pleased to hear you would like to test ab28484 in IHC. This code will give you $325 off your next order before the expiration date. To redeem this offer, please submit an Abreview for IHC and include this code in the “Additional Co...

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Thank you for your reply.

Does the antibody still work in IHC? The reason I ask is that the last order from Cornell for this product is from September 2011. We do offer testing discounts for customers interested in testing our products in unt...

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Thank you for contacting us.

The only other anti-lubricin antibody that we carry is ab94933 which is tested in IHC-P and WB.

http://www.abcam.com/lubricin-antibody-ab94933.html

Unfortunately we do not carry an anti-lubri...

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I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab28484 with the order number 1039188.
To check the status of th...

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Thank you for you enquiry. I have contacted the lab which supplies ab28484 and unfortunately the blocking peptide for that antibody is not available. Instead of using a blocking peptide for a negative control, I would recommend a sample ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample African Green Monkey Cell (COS-1 line, from african green monkey kidney)
Specification COS-1 line, from african green monkey kidney
Fixative Paraformaldehyde
Permeabilization Yes - 0.25% Triton X-100
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
Username

Ms. Melinda Stivers

Verified customer

Submitted Sep 23 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"