Overview

  • Product name
  • Description
    Rabbit polyclonal to Lumican
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 179-228 (AFKGLKSLEY LDLSFNQIAR LPSGLPVSLL TLYLDNNKIS NIPDEYFKRF) of Human Lumican (NP_002336).

  • Positive control
    • Human fetal liver tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab108286 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 38 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Tissue specificity
    Cornea and other tissues.
  • Sequence similarities
    Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class II subfamily.
    Contains 11 LRR (leucine-rich) repeats.
    Contains 1 LRRNT domain.
  • Developmental stage
    Present in the extracellular matrix of human articular cartilage at all ages, although its abundance is far greater in the adult. In the adult cartilage lumican exists predominantly in a glycoprotein form lacking keratan sulfate, whereas the juvenile form of the molecule is a proteoglycan.
  • Post-translational
    modifications
    Sulfated on tyrosine residue(s).
  • Cellular localization
    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • Keratan sulfate proteoglycan lumican antibody
    • KSPG lumican antibody
    • LDC antibody
    • LUM antibody
    • LUM_HUMAN antibody
    • Lumican antibody
    • Lumican precursor antibody
    • SLRR2D antibody
    see all

Anti-Lumican antibody images

  • Anti-Lumican antibody (ab108286) at 1 µg/ml + Human fetal heart tissue lysate at 10 µg

    Predicted band size : 38 kDa

References for Anti-Lumican antibody (ab108286)

ab108286 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Mouse retina, RPE, and eye cup homogenates)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
20 µg
Specification
Mouse retina, RPE, and eye cup homogenates
Blocking step
Licor Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Dec 12 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Tissue lysate - whole (Retinal pigment epithelium, retina)
Gel Running Conditions
Reduced Denaturing (10% gel)
Loading amount
20 µg
Specification
Retinal pigment epithelium, retina
Blocking step
Licor Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Dec 12 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up