Overview

  • Product name
  • Description
    Rabbit polyclonal to Lumican
  • Tested applications
    Suitable for: WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rabbit, Horse, Chicken, Cow
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Human Lumican.

  • Positive control
    • This antibody gave a positive signal in the following whole cell lysates: Liver (Human) Tissue Lysate. This antibody gave a positive signal in the following formaldehyde fixed cell lines: HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab98067 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).
ELISA Use a concentration of 1 µg/ml.
ICC/IF Use a concentration of 5 µg/ml.

Target

  • Tissue specificity
    Cornea and other tissues.
  • Sequence similarities
    Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class II subfamily.
    Contains 11 LRR (leucine-rich) repeats.
    Contains 1 LRRNT domain.
  • Developmental stage
    Present in the extracellular matrix of human articular cartilage at all ages, although its abundance is far greater in the adult. In the adult cartilage lumican exists predominantly in a glycoprotein form lacking keratan sulfate, whereas the juvenile form of the molecule is a proteoglycan.
  • Post-translational
    modifications
    Sulfated on tyrosine residue(s).
  • Cellular localization
    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • Keratan sulfate proteoglycan lumican antibody
    • KSPG lumican antibody
    • LDC antibody
    • LUM antibody
    • LUM_HUMAN antibody
    • Lumican antibody
    • Lumican precursor antibody
    • SLRR2D antibody
    see all

Images

  • All lanes : Anti-Lumican antibody (ab98067) at 1 µg/ml

    Lane 1 : Human liver tissue lysate - total protein (ab29889)
    Lane 2 : Lung (Human) Tissue Lysate
    Lane 3 : Human colon tissue lysate - total protein (ab30051)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 38 kDa
    Observed band size : 38 kDa
    Additional bands at : 50 kDa (possible glycosylated form),60 kDa (possible glycosylated form).

    Exposure time : 8 minutes
  • ICC/IF image of ab98067 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab98067 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:
  • Gu G  et al. Lumican as a novel potential clinical indicator for acute aortic dissection: A comparative study, based on multi-slice computed tomography angiography. Exp Ther Med 11:923-928 (2016). Read more (PubMed: 26998013) »

See 1 Publication for this product

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry
Sample
Mouse Cultured Cells (Oligodendrocyte precursor cell line)
Permeabilization
Yes - 0.5%
Specification
Oligodendrocyte precursor cell line
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Fixative
Paraformaldehyde
Username

Dr. Sueli Marques

Verified customer

Submitted Jan 12 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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