Overview

  • Product nameLuminescent ATP Detection Assay Kit
    See all ATP kits
  • Detection methodLuminescent
  • Assay typeQuantitative
  • Assay time
    0h 30m
  • Assay durationMultiple steps standard assay
  • Product overview

    Luminescent ATP Detection Assay Kit (ab113849) irreversibly inactivates ATP degrading enzymes (ATPases) during the lysis step, ensuring that the luminescent signal obtained truly corresponds to the endogenous levels of ATP. 

     

     

     

  • Notes

    Total levels of cellular ATP can be used to assess cell viability, cell proliferation and cytotoxicity of a wide range of compounds and biological response modifiers.

  • Tested applicationsSuitable for: Functional Studiesmore details
  • PlatformReagents

Properties

  • Alternative names
    • Adenosine 5' triphosphate

Associated products

Applications

Our Abpromise guarantee covers the use of ab113849 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent concentration.

Luminescent ATP Detection Assay Kit images

  • The ATP standard curve was prepared as described in the protocol. Background-subtracted data values (mean +/- SD) are graphed.

  • The ATP standard curve was prepared as described in the protocol. Background-subtracted data values (mean +/- SD) are graphed.

  • ab113849 ATP detection kit cytotoxicity data. 25000 HepG2 cells were seeded into each well, allowed to adhere and treated for 4 hours with 25µM rotenone and vehicle control (DMSO) in glucose based complete media. After treatment, cells were lysed, exposed to the ATP substrate solution and signal was measured on a luminescent counter. Mean and standard deviation is plotted for 3 replicates from each condition. Rotenone induces cytotoxicity in HepG2 cells.
  • Cellular Energy Flux for HepG2 cells (seeded at 65,000 per well), treated with a combination of drug compounds modulating the ETC (Antimycin A [1 µM] and FCCP [2.5 µM]), shown as a percentage relative to untreated control cells. Comparative measurements were taken with Extracellular Oxygen Consumption Assay (ab197243) (white column) and Glycolysis Assay [Extracellular acidification] (ab197244) (black column) show the shift between mitochondrial respiration and glycolysis and the cellular control of energy (ATP; measured 1h post-treatment using Luminescent ATP Detection Assay kit (ab113849) (striped column)).

Protocols

References for Luminescent ATP Detection Assay Kit (ab113849)

This product has been referenced in:
  • Zhou X  et al. 1,25-Dihydroxyvitamin D inhibits glutamine metabolism in Harvey-ras transformed MCF10A human breast epithelial cell. J Steroid Biochem Mol Biol 163:147-56 (2016). Read more (PubMed: 27154413) »
  • Pertega-Gomes N  et al. A glycolytic phenotype is associated with prostate cancer progression and aggressiveness: a role for monocarboxylate transporters as metabolic targets for therapy. J Pathol 236:517-30 (2015). Functional Studies ; Human . Read more (PubMed: 25875424) »

See all 3 Publications for this product

Product Wall


In theory it should work, but we don’t know for sure as we have not tested it. Therefore, if you are willing to test and publish the data in the form of review on our website then I would like to invite you to our Abtrial programme.

Thank you for your enquiry.

Luminescence does not have multiple wavelengths. In Luminescence mode, the instrument simply measures the emission of visible light from the well. What is key in the measurement is the PMT (photomultiplier time). Th...

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The cells do not need to rest or adhere for this assay, you can measure right away.



Any anionic detergent will inhibit the luciferase reaction. What is interesting is that non-ionic detergents have the opposite effect and can in fact enhance the luminescent signal. The following detergents used between 0.5 and 1.5% can enhance the sig...

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SDS is a very strong inhibitor of the luciferase reaction. The lowest possible concentration will likely be below 0.05%. We suggest the customer running a standard ATP curve with and w/o SDS to determine empirically the effect.

Theoretically,...

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The mammalian cell lysis solution contains 0.1M of alkaline solution. I don't think it will be compatible with protein quantification. Indeed, changes in ATP may be due to unevenness in seeding or low viability. Using multiple replicates per each dat...

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I contacted the laboratory to have more information about the standardization of the results obtained with the ab113849 but unfortunately the lab does not have this kind of information. I am very sorry for this inconvenience.


However, fro...

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I have looked into this further and can confirm that in testing clear plates have been used with no major issues. However if you do have black plates, that may be more optimal.

Selon the protocole, p.8, si vous travaillez sur des cellules HELA vous devez cultiver 12,000 cellules par puits. Pour des cellules HepG2, cultivé 25,000 cellules par puits, pour des cellules HdFn, cultivé 12,000 cellules par puits et...

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If they don’t see a change in ATP, this is probably because the cells are switching to glycolysis. My suggestion would be to do the treatment in the presence of galactose/glutamine as carbon substrates rather than glucose/glutamine. Below you wil...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"