Overview

  • Product nameAnti-Lunatic Fringe antibody
    See all Lunatic Fringe primary antibodies
  • Description
    Rabbit polyclonal to Lunatic Fringe
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Guinea pig, Cow, Saccharomyces cerevisiae
  • Immunogen

    Synthetic peptide corresponding to a region of human Lunatic Fringe

  • Positive control
    • Fetal muscle lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab80439 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 42 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/62500.

Target

  • FunctionGlycosyltransferase that initiates the elongation of O-linked fucose residues attached to EGF-like repeats in the extracellular domain of Notch molecules. Decreases the binding of JAGGED1 to NOTCH2 but not that of DELTA1. Essential mediator of somite segmentation and patterning.
  • Involvement in diseaseDefects in LFNG are the cause of spondylocostal dysostosis type 3 (SCDO3) [MIM:609813]. An autosomal recessive condition of variable severity associated with vertebral and rib segmentation defects. The main skeletal malformations include fusion of vertebrae, hemivertebrae, fusion of certain ribs, and other rib malformations. Deformity of the chest and spine (severe scoliosis, kyphoscoliosis and lordosis) is a natural consequence of the malformation and leads to a dwarf-like appearance. As the thorax is small, infants frequently have respiratory insufficiency and repeated respiratory infections resulting in life-threatening complications in the first year of life.
  • Sequence similaritiesBelongs to the glycosyltransferase 31 family.
  • Post-translational
    modifications
    A soluble form may be derived from the membrane form by proteolytic processing.
  • Cellular localizationGolgi apparatus membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 3-N-acetylglucosaminyltransferase lunatic fringe antibody
    • Beta-1 antibody
    • Beta-13-N-acetylglucosaminyltransferase lunatic fringe antibody
    • lfng antibody
    • LFNG_HUMAN antibody
    • O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase antibody
    • SCDO3 antibody
    see all

Anti-Lunatic Fringe antibody images

  • Anti-Lunatic Fringe antibody (ab80439) at 1 µg/ml + fetal muscle lysate at 10 µg

    Secondary
    HRP conjugated anti Rabbit IgG at 1/50000 dilution

    Predicted band size : 42 kDa
    Observed band size : 36 kDa (why is the actual band size different from the predicted?)

References for Anti-Lunatic Fringe antibody (ab80439)

ab80439 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab80439.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"