Anti-Lunatic Fringe antibody [EPR10391(B)] (ab151699)
- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-Lunatic Fringe antibody [EPR10391(B)]
See all Lunatic Fringe primary antibodies -
DescriptionRabbit monoclonal [EPR10391(B)] to Lunatic Fringe
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Host speciesRabbit
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Tested applicationsSuitable for: WB, Flow Cytmore details
Unsuitable for: ICC,IHC-P or IP -
Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic peptide, corresponding to residues in Human Lunatic Fringe (UniProt: Q8NES3).
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Positive control
- PC12, HeLa and K562 cell lysates; K562 cells.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at -20ºC.
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Storage bufferPreservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEPR10391(B)
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IsotypeIgG
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Research areas
Associated products
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab151699 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 42 kDa. | |
| Flow Cyt | 1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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Target
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FunctionGlycosyltransferase that initiates the elongation of O-linked fucose residues attached to EGF-like repeats in the extracellular domain of Notch molecules. Decreases the binding of JAGGED1 to NOTCH2 but not that of DELTA1. Essential mediator of somite segmentation and patterning.
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Involvement in diseaseDefects in LFNG are the cause of spondylocostal dysostosis type 3 (SCDO3) [MIM:609813]. An autosomal recessive condition of variable severity associated with vertebral and rib segmentation defects. The main skeletal malformations include fusion of vertebrae, hemivertebrae, fusion of certain ribs, and other rib malformations. Deformity of the chest and spine (severe scoliosis, kyphoscoliosis and lordosis) is a natural consequence of the malformation and leads to a dwarf-like appearance. As the thorax is small, infants frequently have respiratory insufficiency and repeated respiratory infections resulting in life-threatening complications in the first year of life.
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Sequence similaritiesBelongs to the glycosyltransferase 31 family.
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Post-translational
modificationsA soluble form may be derived from the membrane form by proteolytic processing. -
Cellular localizationGolgi apparatus membrane.
- Information by UniProt
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Database links
- Entrez Gene: 3955 Human
- Entrez Gene: 16848 Mouse
- Entrez Gene: 170905 Rat
- Omim: 602576 Human
- SwissProt: Q8NES3 Human
- SwissProt: O09010 Mouse
- SwissProt: Q924T4 Rat
- Unigene: 159142 Human
see all -
Alternative names
- 3-N-acetylglucosaminyltransferase lunatic fringe antibody
- Beta-1 antibody
- Beta-13-N-acetylglucosaminyltransferase lunatic fringe antibody
see all
Images
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Western blot - (ab151699)All lanes : Anti-Lunatic Fringe antibody [EPR10391(B)] (ab151699) at 1/1000 dilution
Lane 1 : PC12 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : K562 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 42 kDa -
Flow Cytometry - (ab151699)Flow cytometric analysis of permeabilized K562 cells labeling Lunatic Fringe with ab151699 at 1/10 dilution (red) compared to a nonspecific control antibody (green).
References
ab151699 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"