Overview

  • Product name
  • Description
    Rabbit polyclonal to LXN
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Cow
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 1-222 of Human LXN (UniProt ID: Q9BS40).

  • Positive control
    • 293T, HeLa S3, Molt4 and mouse brain lysates; mouse embryonic stem cells D3; Human FaDu xenograft.

Properties

Applications

Our Abpromise guarantee covers the use of ab154744 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 26 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Hardly reversible, non-competitive, and potent inhibitor of CPA1, CPA2 and CPA4.
  • Tissue specificity
    Highly expressed in heart, prostate, ovary, kidney, pancreas, and colon, moderate or low in other tissues including brain.
  • Sequence similarities
    Belongs to the protease inhibitor I47 (latexin) family.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • ECI antibody
    • Endogenous carboxypeptidase inhibitor antibody
    • Latexin antibody
    • Latexin protein antibody
    • LXN antibody
    • LXN_HUMAN antibody
    • MUM antibody
    • Protein MUM antibody
    • TCI antibody
    • Tissue carboxypeptidase inhibitor antibody
    see all

Images

  • All lanes : Anti-LXN antibody (ab154744) at 1/1000 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : HeLa S3 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 26 kDa



    12% SDS PAGE
  • Anti-LXN antibody (ab154744) at 1/1000 dilution + mouse brain whole cell lysate at 50 µg

    Predicted band size: 26 kDa



    12% SDS PAGE
  • Immunofluorescent analysis of paraformaldehyde-fixed mouse embryonic stem cells D3 labeling LXN with ab154744 at 1/200 dilution. Lower panel is co-stained with Hoechst 33342.
  • Immunohistochemical analysis of paraffin-embedded FaDu xenograft labeling LXN with ab154744 at 1/500 dilution.

References

ab154744 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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