- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-LXN antibody
See all LXN primary antibodies -
DescriptionGoat polyclonal to LXN
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Host speciesGoat
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Tested applicationsSuitable for: IHC-P, WBmore details
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Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Cow, Dog
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Immunogen
Synthetic peptide:
C-QYGTKVKHNSR
, corresponding to C terminal amino acids 204-214 of Human LXN -
Positive control
- Human Brain (Cerebellum), Mouse Brain and Rat Brain lysates.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3 -
Concentration information loading... -
PurityImmunogen affinity purified
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Purification notesab59521 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab59521 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use at an assay dependent concentration. | |
| WB | Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 25 kDa). |
Target
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FunctionHardly reversible, non-competitive, and potent inhibitor of CPA1, CPA2 and CPA4.
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Tissue specificityHighly expressed in heart, prostate, ovary, kidney, pancreas, and colon, moderate or low in other tissues including brain.
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Sequence similaritiesBelongs to the protease inhibitor I47 (latexin) family.
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Cellular localizationCytoplasm.
- Information by UniProt
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Database links
- Entrez Gene: 610062 Dog
- Entrez Gene: 56925 Human
- Entrez Gene: 17035 Mouse
- Entrez Gene: 59073 Rat
- Omim: 609305 Human
- SwissProt: Q9BS40 Human
- SwissProt: P70202 Mouse
- SwissProt: Q64361 Rat
see all -
Alternative names
- ECI antibody
- Endogenous carboxypeptidase inhibitor antibody
- Latexin antibody
see all
Images
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Western blot - Anti-LXN antibody (ab59521)Anti-LXN antibody (ab59521) at 0.1 µg/ml + rat brain lysate in RIPA buffer at 35 µg
Predicted band size: 25 kDa
Observed band size: 30 kDa (why is the actual band size different from the predicted?)
Detected by chemiluminescence.
References
ab59521 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"