Our Abpromise guarantee covers the use of ab28478 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Predicted molecular weight: 50 kDa.


  • FunctionOrphan receptor. Interaction with RXR shifts RXR from its role as a silent DNA-binding partner to an active ligand-binding subunit in mediating retinoid responses through target genes defined by LXRES. LXRES are DR4-type response elements characterized by direct repeats of two similar hexanuclotide half-sites spaced by four nucleotides. Plays an important role in the regulation of cholesterol homeostasis, regulating cholesterol uptake through MYLIP-dependent ubiquitination of LDLR, VLDLR and LRP8.
  • Tissue specificityVisceral organs specific expression. Strong expression was found in liver, kidney and intestine followed by spleen and to a lesser extent the adrenals.
  • Sequence similaritiesBelongs to the nuclear hormone receptor family. NR1 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Liver X receptor alpha antibody
    • LXR a antibody
    • LXRA antibody
    • NR1H3 antibody
    • NR1H3_HUMAN antibody
    • Nuclear receptor subfamily 1 group H member 3 antibody
    • Oxysterols receptor LXR alpha antibody
    • Oxysterols receptor LXR-alpha antibody
    • RLD 1 antibody
    • RLD1 antibody
    see all

Anti-LXR alpha antibody images

  • Anti-LXR alpha antibody (ab28478) (at a dilution of 1/500) + mouse liver samples

    Predicted band size : 50 kDa
    Observed band size : 50 kDa

References for Anti-LXR alpha antibody (ab28478)

This product has been referenced in:
  • Sharma M  et al. Lipoprotein (a) upregulates ABCA1 in liver cells via scavenger receptor-B1 through its oxidized phospholipids. J Lipid Res 56:1318-28 (2015). Read more (PubMed: 25852127) »
  • Pirinen E  et al. Activated polyamine catabolism leads to low cholesterol levels by enhancing bile acid synthesis. Amino Acids 38:549-60 (2010). WB ; Mouse . Read more (PubMed: 19956992) »

See all 4 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HepG2, 293T, MEF)
Loading amount 20 µg
Specification HepG2, 293T, MEF
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 7% · Temperature: 20°C

Mr. Dongil Kim

Verified customer

Submitted Feb 01 2013

Thank you for taking the time to contact us. I am sorry to hear the customer has some concerns regarding the results from this antibody.

In answer to your question, the alignment of the immunogen of ab28478 with beta subunit is 26%. So it is...

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I'm sorry to hear you have been experiencing problems with ab28478 in western blotting. Thank you for providing your protocol details which enabled me to understand rapidly how you performed your experiment. I have a few suggestions detailed below ...

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