Overview

  • Product name
  • Description
    Rabbit polyclonal to LXR alpha
  • Specificity
    Detects recombinant Human LXR alpha. This antibody does not detect recombinant Human LXR beta.
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Chinese hamster
    Predicted to work with: Cow
  • Immunogen

    Synthetic peptide corresponding to Human LXR alpha aa 318-329.
    Sequence:

    DFSYNREDFAKA


    (Peptide available as ab5021)

Properties

Applications

Our Abpromise guarantee covers the use of ab3585 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.
WB 1/300. Can be blocked with LXR alpha peptide (ab5021).
IHC-FoFr Use at an assay dependent concentration. PubMed: 22836489
EMSA Use at an assay dependent concentration.

Target

  • Function
    Orphan receptor. Interaction with RXR shifts RXR from its role as a silent DNA-binding partner to an active ligand-binding subunit in mediating retinoid responses through target genes defined by LXRES. LXRES are DR4-type response elements characterized by direct repeats of two similar hexanuclotide half-sites spaced by four nucleotides. Plays an important role in the regulation of cholesterol homeostasis, regulating cholesterol uptake through MYLIP-dependent ubiquitination of LDLR, VLDLR and LRP8.
  • Tissue specificity
    Visceral organs specific expression. Strong expression was found in liver, kidney and intestine followed by spleen and to a lesser extent the adrenals.
  • Sequence similarities
    Belongs to the nuclear hormone receptor family. NR1 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Liver X receptor alpha antibody
    • LXR a antibody
    • LXRA antibody
    • NR1H3 antibody
    • NR1H3_HUMAN antibody
    • Nuclear receptor subfamily 1 group H member 3 antibody
    • Oxysterols receptor LXR alpha antibody
    • Oxysterols receptor LXR-alpha antibody
    • RLD 1 antibody
    • RLD1 antibody
    see all

Images

  • Immunocytochemistry/Immunofluorescence analysis of MCF-7 cells labeling LXR alpha (green) with ab3585 at 1/300. F-Actin staining with Phalloidin (red) and nuclei with DAPI or Hoechst (blue). Cells were fixed with formalin, permeablized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA in PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody overnight at 4°C. A DyLight-conjugated secondary antibody was used. 60X magnification. Left - negative control.

  • Immunocytochemistry/Immunofluorescence analysis of HeLa cells labeling LXR alpha (green) with ab3585 at 1/300. F-Actin staining with Phalloidin (red) and nuclei with DAPI or Hoechst (blue). Cells were fixed with formalin, permeablized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA in PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody overnight at 4°C. A DyLight-conjugated secondary antibody was used. 60X magnification. Left - negative control.

  • ab3585 at 1/100 staining hamster CHO-K1 cells expressing human LXRa by ICC/IF. The cells were formaldehyde fixed, permeabilized and blocked before being stained with the antibody for 1 hour at 22°C. A FITC labelled goat anti-rabbit antibody was used as the secondary.

    See Abreview

  • Anti-LXR alpha antibody (ab3585) at 1/1000 dilution + Whole cell lysate prepared from Chinese hamster CHO cells at 50000 cells

    Secondary
    HRP conjugated goat polyclonal to rabbit Ig at 1/1000 dilution

    Performed under reducing conditions.

    Observed band size : 50 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute

    This image is courtesy of an anonymous abreview.

    See Abreview

References

This product has been referenced in:
  • Luo L & Yang T Triptolide inhibits the progression of atherosclerosis in apolipoprotein E(-)/(-) mice. Exp Ther Med 12:2307-2313 (2016). WB ; Mouse . Read more (PubMed: 27698729) »
  • Yu R  et al. Baicalin promotes cholesterol efflux by regulating the expression of SR-BI in macrophages. Exp Ther Med 12:4113-4120 (2016). Read more (PubMed: 28105139) »

See all 10 Publications for this product

Customer reviews and Q&As



The lab has tested the antibody at a 1:300 dilution thus it is our recommended dilution, however ab3585 has been used successfully at a 1:1000 dilution by another user who submitted an Abreview with their protocol and data (the image that you...

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Merci de nous avoir contactés.

Garantie sur les produits Abcam: nos produitssont garantis pour fonctionner tels qu'ils sont décrits sur la fiche technique (garantie Abpromise). Nous pouvons donc toujours résoudre un probl...

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Muchas gracias por contactarnos de nuevo.
Efectivamente el péptido usado para producir ab3585 es ab5021, y por tanto puede usarse en los ensayos de bloqueo del mismo. Sin embargo no hay ninguna publicación disponible acerca de este ensayo de bloqu...

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Thank you for your inquiry.

All of the information we have for each of our products is listed on the online datasheet for your convenience. These are updated as soon as any new information is brought to our attention. Therefore, unfortunatel...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Chinese Hamster Cell lysate - whole cell (CHO cell lysate)
Loading amount
50000 cells
Specification
CHO cell lysate
Gel Running Conditions
Reduced Denaturing
Blocking step
(agent) for 30 minute(s) · Concentration: 100% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Nov 21 2008

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa transiently expressing human LXRa.)
Specification
HeLa transiently expressing human LXRa.
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% TritonX-100 in PBS for 10 min at 4'C
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 0.1% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted May 21 2008

Application
Immunocytochemistry/ Immunofluorescence
Sample
Hamster Cell (CHO-K1 cells expressing Human LXRa)
Specification
CHO-K1 cells expressing Human LXRa
Fixative
Formaldehyde
Permeabilization
Yes - 0.1 % Triton X-100
Blocking step
TBS + 3% Dried Milk + 0.1 % Triton X-100 as blocking agent for 20 hour(s) and 0 minute(s) · Concentration: 3%
Username

Dr. Mahesh Mathrubutham

Verified customer

Submitted Aug 21 2007

Thank you for your enquiry. I am sorry you are experiencing problems with this antibody. Often it is possible to make suggestions to improve your results. If we determine that the antibody is not working as stated on the datasheet, and you have pur...

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Thank you for your email. For both of these antibodies, ab3585 and ab24361, they have only so been tested for cross-reactivity with human and have not yet been tested in other species. Ab24361 is predicted to react with Mouse (85% identity with immunog...

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