Overview

  • Product nameAnti-LXR alpha antibody
    See all LXR alpha primary antibodies
  • Description
    Rabbit polyclonal to LXR alpha
  • SpecificityDetects recombinant Human LXR alpha. This antibody does not detect recombinant Human LXR beta.
  • Tested applicationsSuitable for: ICC/IF, WB, Gel supershift assays, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Chinese Hamster
    Predicted to work with: Cow
  • Immunogen

    Synthetic peptide corresponding to Human LXR alpha aa 318-329.
    Sequence:

    DFSYNREDFAKA


    (Peptide available as ab5021)

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab3585 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.
WB 1/300. Can be blocked with LXR alpha peptide (ab5021).
Gel supershift assays Use at an assay dependent concentration.
IHC-FoFr Use at an assay dependent concentration. PubMed: 22836489

Target

  • FunctionOrphan receptor. Interaction with RXR shifts RXR from its role as a silent DNA-binding partner to an active ligand-binding subunit in mediating retinoid responses through target genes defined by LXRES. LXRES are DR4-type response elements characterized by direct repeats of two similar hexanuclotide half-sites spaced by four nucleotides. Plays an important role in the regulation of cholesterol homeostasis, regulating cholesterol uptake through MYLIP-dependent ubiquitination of LDLR, VLDLR and LRP8.
  • Tissue specificityVisceral organs specific expression. Strong expression was found in liver, kidney and intestine followed by spleen and to a lesser extent the adrenals.
  • Sequence similaritiesBelongs to the nuclear hormone receptor family. NR1 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Liver X receptor alpha antibody
    • LXR a antibody
    • LXRA antibody
    • NR1H3 antibody
    • NR1H3_HUMAN antibody
    • Nuclear receptor subfamily 1 group H member 3 antibody
    • Oxysterols receptor LXR alpha antibody
    • Oxysterols receptor LXR-alpha antibody
    • RLD 1 antibody
    • RLD1 antibody
    see all

Anti-LXR alpha antibody images

  • Immunocytochemistry/Immunofluorescence analysis of MCF-7 cells labeling LXR alpha (green) with ab3585 at 1/300. F-Actin staining with Phalloidin (red) and nuclei with DAPI or Hoechst (blue). Cells were fixed with formalin, permeablized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA in PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody overnight at 4°C. A DyLight-conjugated secondary antibody was used. 60X magnification. Left - negative control.

  • Immunocytochemistry/Immunofluorescence analysis of HeLa cells labeling LXR alpha (green) with ab3585 at 1/300. F-Actin staining with Phalloidin (red) and nuclei with DAPI or Hoechst (blue). Cells were fixed with formalin, permeablized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA in PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody overnight at 4°C. A DyLight-conjugated secondary antibody was used. 60X magnification. Left - negative control.

  • ab3585 at 1/100 staining hamster CHO-K1 cells expressing human LXRa by ICC/IF. The cells were formaldehyde fixed, permeabilized and blocked before being stained with the antibody for 1 hour at 22°C. A FITC labelled goat anti-rabbit antibody was used as the secondary.

    See Abreview

  • Anti-LXR alpha antibody (ab3585) at 1/1000 dilution + Whole cell lysate prepared from Chinese hamster CHO cells at 50000 cells

    Secondary
    HRP conjugated goat polyclonal to rabbit Ig at 1/1000 dilution

    Performed under reducing conditions.

    Observed band size : 50 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute

    This image is a courtsey of anonymous abreview.

    See Abreview

References for Anti-LXR alpha antibody (ab3585)

This product has been referenced in:
  • Dasari B  et al. Differential effects of the estrogen receptor agonist estradiol on toxicity induced by enzymatically-derived or autoxidation-derived oxysterols in human ARPE-19 cells. Curr Eye Res 38:1159-71 (2013). WB . Read more (PubMed: 23841471) »
  • García-Mediavilla MV  et al. Liver X receptor a-mediated regulation of lipogenesis by core and NS5A proteins contributes to HCV-induced liver steatosis and HCV replication. Lab Invest : (2012). WB . Read more (PubMed: 22641099) »

See all 6 Publications for this product

Product Wall



The lab has tested the antibody at a 1:300 dilution thus it is our recommended dilution, however ab3585 has been used successfully at a 1:1000 dilution by another user who submitted an Abreview with their protocol and data (the image that you...

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Merci de nous avoir contactés.

Garantie sur les produits Abcam: nos produitssont garantis pour fonctionner tels qu'ils sont décrits sur la fiche technique (garantie Abpromise). Nous pouvons donc toujours résoudre un probl...

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Muchas gracias por contactarnos de nuevo.
Efectivamente el péptido usado para producir ab3585 es ab5021, y por tanto puede usarse en los ensayos de bloqueo del mismo. Sin embargo no hay ninguna publicación disponible acerca de este ensayo de bloqu...

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Thank you for your inquiry.

All of the information we have for each of our products is listed on the online datasheet for your convenience. These are updated as soon as any new information is brought to our attention. Therefore, unfortunatel...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Chinese Hamster Cell lysate - whole cell (CHO cell lysate)
Loading amount 50000 cells
Specification CHO cell lysate
Gel Running Conditions Reduced Denaturing
Blocking step (agent) for 30 minute(s) · Concentration: 100% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Nov 21 2008

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa transiently expressing human LXRa.)
Specification HeLa transiently expressing human LXRa.
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% TritonX-100 in PBS for 10 min at 4'C
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 0.1% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted May 21 2008

Application Immunocytochemistry/ Immunofluorescence
Sample Hamster Cell (CHO-K1 cells expressing Human LXRa)
Specification CHO-K1 cells expressing Human LXRa
Fixative Formaldehyde
Permeabilization Yes - 0.1 % Triton X-100
Blocking step TBS + 3% Dried Milk + 0.1 % Triton X-100 as blocking agent for 20 hour(s) and 0 minute(s) · Concentration: 3%
Username

Dr. Mahesh Mathrubutham

Verified customer

Submitted Aug 21 2007

Thank you for your enquiry. I am sorry you are experiencing problems with this antibody. Often it is possible to make suggestions to improve your results. If we determine that the antibody is not working as stated on the datasheet, and you have pur...

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Thank you for your email. For both of these antibodies, ab3585 and ab24361, they have only so been tested for cross-reactivity with human and have not yet been tested in other species. Ab24361 is predicted to react with Mouse (85% identity with immunog...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"