Overview

  • Product name
  • Description
    Rabbit polyclonal to LXR alpha
  • Specificity
    Detects recombinant Human LXR alpha. This antibody does not detect recombinant Human LXR beta.
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Chinese hamster
    Predicted to work with: Cow
  • Immunogen

    Synthetic peptide corresponding to Human LXR alpha aa 318-329.
    Sequence:

    DFSYNREDFAKA


    (Peptide available as ab5021)

Properties

Applications

Our Abpromise guarantee covers the use of ab3585 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.
WB 1/300. Can be blocked with LXR alpha peptide (ab5021).
IHC-FoFr Use at an assay dependent concentration. PubMed: 22836489
EMSA Use at an assay dependent concentration.

Target

  • Function
    Orphan receptor. Interaction with RXR shifts RXR from its role as a silent DNA-binding partner to an active ligand-binding subunit in mediating retinoid responses through target genes defined by LXRES. LXRES are DR4-type response elements characterized by direct repeats of two similar hexanuclotide half-sites spaced by four nucleotides. Plays an important role in the regulation of cholesterol homeostasis, regulating cholesterol uptake through MYLIP-dependent ubiquitination of LDLR, VLDLR and LRP8.
  • Tissue specificity
    Visceral organs specific expression. Strong expression was found in liver, kidney and intestine followed by spleen and to a lesser extent the adrenals.
  • Sequence similarities
    Belongs to the nuclear hormone receptor family. NR1 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Liver X receptor alpha antibody
    • LXR a antibody
    • LXRA antibody
    • NR1H3 antibody
    • NR1H3_HUMAN antibody
    • Nuclear receptor subfamily 1 group H member 3 antibody
    • Oxysterols receptor LXR alpha antibody
    • Oxysterols receptor LXR-alpha antibody
    • RLD 1 antibody
    • RLD1 antibody
    see all

Images

  • Immunocytochemistry/Immunofluorescence analysis of MCF-7 cells labeling LXR alpha (green) with ab3585 at 1/300. F-Actin staining with Phalloidin (red) and nuclei with DAPI or Hoechst (blue). Cells were fixed with formalin, permeablized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA in PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody overnight at 4°C. A DyLight-conjugated secondary antibody was used. 60X magnification. Left - negative control.

  • Immunocytochemistry/Immunofluorescence analysis of HeLa cells labeling LXR alpha (green) with ab3585 at 1/300. F-Actin staining with Phalloidin (red) and nuclei with DAPI or Hoechst (blue). Cells were fixed with formalin, permeablized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA in PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody overnight at 4°C. A DyLight-conjugated secondary antibody was used. 60X magnification. Left - negative control.

  • ab3585 at 1/100 staining hamster CHO-K1 cells expressing human LXRa by ICC/IF. The cells were formaldehyde fixed, permeabilized and blocked before being stained with the antibody for 1 hour at 22°C. A FITC labelled goat anti-rabbit antibody was used as the secondary.

    See Abreview

  • Anti-LXR alpha antibody (ab3585) at 1/1000 dilution + Whole cell lysate prepared from Chinese hamster CHO cells at 50000 cells

    Secondary
    HRP conjugated goat polyclonal to rabbit Ig at 1/1000 dilution

    Performed under reducing conditions.

    Observed band size : 50 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute

    This image is courtesy of an anonymous abreview.

    See Abreview

References

This product has been referenced in:
  • Luo L & Yang T Triptolide inhibits the progression of atherosclerosis in apolipoprotein E(-)/(-) mice. Exp Ther Med 12:2307-2313 (2016). WB ; Mouse . Read more (PubMed: 27698729) »
  • Yu R  et al. Baicalin promotes cholesterol efflux by regulating the expression of SR-BI in macrophages. Exp Ther Med 12:4113-4120 (2016). Read more (PubMed: 28105139) »

See all 10 Publications for this product

Customer reviews and Q&As

Filter by Application

Filter by Species

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Chinese Hamster Cell lysate - whole cell (CHO cell lysate)
Loading amount
50000 cells
Specification
CHO cell lysate
Gel Running Conditions
Reduced Denaturing
Blocking step
(agent) for 30 minute(s) · Concentration: 100% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Nov 21 2008

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa transiently expressing human LXRa.)
Specification
HeLa transiently expressing human LXRa.
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% TritonX-100 in PBS for 10 min at 4'C
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 0.1% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted May 21 2008

Application
Immunocytochemistry/ Immunofluorescence
Sample
Hamster Cell (CHO-K1 cells expressing Human LXRa)
Specification
CHO-K1 cells expressing Human LXRa
Fixative
Formaldehyde
Permeabilization
Yes - 0.1 % Triton X-100
Blocking step
TBS + 3% Dried Milk + 0.1 % Triton X-100 as blocking agent for 20 hour(s) and 0 minute(s) · Concentration: 3%
Username

Dr. Mahesh Mathrubutham

Verified customer

Submitted Aug 21 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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