Anti-LXR alpha antibody [PPZ0412] - ChIP Grade (ab41902)

Overview

  • Product name
    Anti-LXR alpha antibody [PPZ0412] - ChIP Grade
    See all LXR alpha primary antibodies
  • Description
    Mouse monoclonal [PPZ0412] to LXR alpha - ChIP Grade
  • Specificity
    This antibody does not recognize human LXR beta.
  • Tested applications
    Suitable for: ChIP, ICC/IF, WB, ELISA, IP, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    E.coli-expressed recombinant fragment, corresponding to amino acids 164-447 of Human LXR alpha.

  • Positive control
    • Rat hepatocyte and kupffer cell. Rat spleen macrophage.

Applications

Our Abpromise guarantee covers the use of ab41902 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ChIP Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
EMSA Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 50 kDa.
ELISA Use a concentration of 0.2 µg/ml.
IP Use at an assay dependent concentration.
IHC-P Use a concentration of 20 - 40 µg/ml.
Flow Cyt Use 1µg for 106 cells.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Orphan receptor. Interaction with RXR shifts RXR from its role as a silent DNA-binding partner to an active ligand-binding subunit in mediating retinoid responses through target genes defined by LXRES. LXRES are DR4-type response elements characterized by direct repeats of two similar hexanuclotide half-sites spaced by four nucleotides. Plays an important role in the regulation of cholesterol homeostasis, regulating cholesterol uptake through MYLIP-dependent ubiquitination of LDLR, VLDLR and LRP8.
  • Tissue specificity
    Visceral organs specific expression. Strong expression was found in liver, kidney and intestine followed by spleen and to a lesser extent the adrenals.
  • Sequence similarities
    Belongs to the nuclear hormone receptor family. NR1 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Liver X receptor alpha antibody
    • LXR a antibody
    • LXRA antibody
    • NR1H3 antibody
    • NR1H3_HUMAN antibody
    • Nuclear receptor subfamily 1 group H member 3 antibody
    • Oxysterols receptor LXR alpha antibody
    • Oxysterols receptor LXR-alpha antibody
    • RLD 1 antibody
    • RLD1 antibody
    see all

Images

  • Cross-linking (X-ChIP) analysis of RAW 264.7 murine macrophage cell line nuclear cell lysate labeling LXR alpha with ab41902. 10 minutes duration of cross-linking using formaldehyde as cross-linking agent. Postive control: DMSO treated cells and T091317 treated cells (RAW overexpressing human LXRalpha); nehative control: DMSO treated cells and T091317 treated cells (RAW without overexpression of human LXRalpha, RAW VO). Samples were immunoprecipitated with mouse IgG.

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen tissue sections labeling LXR alpha with ab41902 at 1/100 dilution. Tissue was fixed with formaldehyde and permeabilized with Tween 20. Heat mediated antigen retrieval was performed using TRIS-EDTA Buffer. Tissue sections were incubated with Anti-LXR alpha antibody [PPZ0412] - ChIP Grade (ab41902) for 30 minutes at 20°C in diluent from DAKO. An undiluted HRP conjugated horse anti-mouse secondary antibody was used.

    See Abreview

  • All lanes : Anti-LXR alpha antibody [PPZ0412] - ChIP Grade (ab41902) at 1/1000 dilution

    Lane 1 : Bone marrow-derivate of macrophages from C57Bl6 mice treated with DMSO
    Lane 2 : Bone marrow-derivate of macrophages from C57Bl6 mice treated with T091317

    Lysates/proteins at 40 µg per lane.

    Secondary
    Goant anti-murine IgG HRP (Undiluted)
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 50 kDa


    Exposure time : 5 minutes

    This image is courtesy of an anonymous abreview.

    See Abreview

  • ab41902 staining LXR alpha in rat spleen macrophage cell paraffin section (20-40ug/mL) by Immunohistochemistry, formalin-fixed paraffin embedded sections.
  • Immunocytochemistry/ Immunofluorescence analysis of human THP1 monocyte-derived macrophages labeling LXR alpha with ab41902 at 1/50. Cells were fixed with paraformaldehyde and permeabilized with 0.25% Triton X-100 in PBS. Next the cells were blocked with 10% BSA for 30 minutes at room temperature, followed by incubation with primary antibody in 10% BSA/0.05% Triton/0.3M glycine in PBS for 16 hours at 4°C. A polyclonal donkey anti-mouse Alexa Fluor® 555 secondary antibody was used at 1/300 dilution. DAPI nuclear counter stain.

    See Abreview

  • ab41902 staining LXR alpha in Rat Hepatocyte and Kupffer cell paraffin section (20-40ug/mL) by Immunohistochemistry, formalin-fixed paraffin embedded sections.
  • All lanes : Anti-LXR alpha antibody [PPZ0412] - ChIP Grade (ab41902) at 1/1000 dilution (in 2.5% BSA + TBS for 16 hours at 4°C)

    Lane 1 : RAW 264.7 murine macrophage cell line lysate without endogenous LXRalpha
    Lane 2 : RAW 264.7 murine macrophage cell line lysate overexpressing human FLAG-LXRalpha

    Lysates/proteins at 35 µg per lane.

    Secondary
    An HRP-conjugated Goat anti-mouse IgG polyclonal at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 50 kDa
    Observed band size : 50 kDa


    Exposure time : 1 minute

    This image is courtesy of an anonymous Abreview

    Blocking step: 5% Milk for 1 hour at 25°C

    See Abreview

  • Overlay histogram showing HepG2 cells stained with ab41902 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab41902, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References

This product has been referenced in:
  • Song L  et al. Phytosterol esters attenuate hepatic steatosis in rats with non-alcoholic fatty liver disease rats fed a high-fat diet. Sci Rep 7:41604 (2017). WB ; Rat . Read more (PubMed: 28169366) »
  • Ikhlef S  et al. Human paraoxonase 1 overexpression in mice stimulates HDL cholesterol efflux and reverse cholesterol transport. PLoS One 12:e0173385 (2017). WB ; Mouse . Read more (PubMed: 28278274) »

See all 29 Publications for this product

Customer reviews and Q&As

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Application
Western blot
Sample
Human Cell lysate - whole cell (monocyte-derived macrophage)
Gel Running Conditions
Non-reduced Denaturing
Loading amount
100000 cells
Specification
monocyte-derived macrophage
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jun 29 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HepG2 cells)
Loading amount
30 µg
Specification
HepG2 cells
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 8% · Temperature: 20°C
Username

Mr. Dongil Kim

Verified customer

Submitted Apr 08 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (lung)
Loading amount
30 µg
Specification
lung
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Username

Ms. Jihun Lee

Verified customer

Submitted Jan 10 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Mono-mac 6)
Loading amount
50 µg
Specification
Mono-mac 6
Treatment
TO-901317
Gel Running Conditions
Reduced Denaturing (8%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.5% · Temperature: RT°C
Username

Dr. Elizabeth Kilby

Verified customer

Submitted Dec 19 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (THP1 monocyte-derived macrophages)
Specification
THP1 monocyte-derived macrophages
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.25% Triton X-100 in PBS
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: RT°C
Username

Dr. Elizabeth Kilby

Verified customer

Submitted Oct 17 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Tissue lysate - whole (Rat liver tissue)
Loading amount
120 µg
Specification
Rat liver tissue
Treatment
ovariectomy
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
5% milk in TBS-T(0.1%) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Mrs. Smitha Koshy

Verified customer

Submitted May 21 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Liver)
Loading amount
50 µg
Specification
Liver
Gel Running Conditions
Reduced Denaturing (12.5)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Apr 17 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
ChIP
Sample
Mouse Cell lysate - nuclear (RAW 264.7 murine macrophage cell line)
Specification
RAW 264.7 murine macrophage cell line
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: Formaldehyde
Detection step
Real-time PCR
Positive control
DMSO treated cells and T091317 treated cells (RAW overexpressing human LXRalpha)
Negative control
DMSO treated cells and T091317 treated cells (RAW without overexpression of human LXRalpha, RAW VO)

Samples immunoprecipitated with mouse IgG
Username

Abcam user community

Verified customer

Submitted Jun 30 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Bone Marrow-Derivated Macrophages from C57Bl6 mice)
Loading amount
40 µg
Specification
Bone Marrow-Derivated Macrophages from C57Bl6 mice
Treatment
T091317 ligand for 48 hours
Gel Running Conditions
Reduced Denaturing (12% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jun 25 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (RAW 264.7 murine macrophage cell line)
Loading amount
35 µg
Specification
RAW 264.7 murine macrophage cell line
Gel Running Conditions
Reduced Denaturing (12% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jun 22 2010

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