• Product name
  • Description
    Mouse monoclonal to LXR beta
  • Tested applications
    Suitable for: ELISA, WB, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Recombinant full length human LXR beta protein (AAH07790) with a 26 kDa tag.

  • Positive control
    • IMR-32 cell lysate, HeLa cells
  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by July 2018 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. 



Our Abpromise guarantee covers the use of ab76983 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 51 kDa.
ICC/IF Use a concentration of 10 µg/ml.
Flow Cyt Use 1µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.


  • Function
    Orphan receptor. Binds preferentially to double-stranded oligonucleotide direct repeats having the consensus half-site sequence 5'-AGGTCA-3' and 4-nt spacing (DR-4). Regulates cholesterol uptake through MYLIP-dependent ubiquitination of LDLR, VLDLR and LRP8.
  • Tissue specificity
  • Sequence similarities
    Belongs to the nuclear hormone receptor family. NR1 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • Liver X nuclear receptor beta antibody
    • Liver X receptor beta antibody
    • LX receptor beta antibody
    • LXR b antibody
    • LXR beta antibody
    • LXRB antibody
    • NER antibody
    • NER I antibody
    • NER1 antibody
    • NR1H 2 antibody
    • NR1H2 antibody
    • NR1H2_HUMAN antibody
    • Nuclear orphan receptor LXR beta antibody
    • Nuclear receptor NER antibody
    • Nuclear receptor subfamily 1 group H member 2 antibody
    • OR-1 antibody
    • Oxysterols receptor LXR-beta antibody
    • RIP15 antibody
    • Steroid hormone nuclear receptor NER antibody
    • Ubiquitously-expressed nuclear receptor antibody
    • UNR antibody
    see all


  • Anti-LXR beta antibody (ab76983) at 5 µg/ml + IMR-32 cell lysate at 25 µg

    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 51 kDa
    Observed band size : 51 kDa
  • Immunofluorescence analysis of LXR beta expression in HeLa cells using 10 µg/ml of ab76983
  • Detection limit for ab76983 in Sandwich ELISA (using recombinant protein) is approximately 0.03 ng/ml as a capture antibody.
  • Overlay histogram showing HeLa cells stained with ab76983 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76983, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.


This product has been referenced in:
  • McDonald G  et al. Normalizing glycosphingolipids restores function in CD4+ T cells from lupus patients. J Clin Invest 124:712-24 (2014). Human . Read more (PubMed: 24463447) »
  • Kruse MS  et al. Alterations of LXRa and LXRß expression in the hypothalamus of glucose-intolerant rats. J Endocrinol 215:51-8 (2012). IHC ; Rat . Read more (PubMed: 22836489) »

See all 2 Publications for this product

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We have several antibodies against LXR beta (ab24361, ab56237, ab58089, ab58092, ab76983, ab106473) . The tested application/species/unit size can be found on the on-line product d...

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Western blot
Human Cell lysate - whole cell (THP-1 cells)
Loading amount
35 µg
THP-1 cells
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

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Submitted Dec 07 2009


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