Overview

  • Product nameAnti-LXR beta antibody
    See all LXR beta primary antibodies
  • Description
    Mouse monoclonal to LXR beta
  • Tested applicationsSuitable for: ELISA, WB, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Recombinant full length human LXR beta protein (AAH07790) with a 26 kDa tag.

  • Positive control
    • IMR-32 cell lysate, HeLa cells

Properties

Applications

Our Abpromise guarantee covers the use of ab76983 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 51 kDa.
ICC/IF Use a concentration of 10 µg/ml.
Flow Cyt Use 1µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Target

  • FunctionOrphan receptor. Binds preferentially to double-stranded oligonucleotide direct repeats having the consensus half-site sequence 5'-AGGTCA-3' and 4-nt spacing (DR-4). Regulates cholesterol uptake through MYLIP-dependent ubiquitination of LDLR, VLDLR and LRP8.
  • Tissue specificityUbiquitous.
  • Sequence similaritiesBelongs to the nuclear hormone receptor family. NR1 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Liver X nuclear receptor beta antibody
    • Liver X receptor beta antibody
    • LX receptor beta antibody
    • LXR b antibody
    • LXR beta antibody
    • LXRB antibody
    • NER antibody
    • NER I antibody
    • NER1 antibody
    • NR1H 2 antibody
    • NR1H2 antibody
    • NR1H2_HUMAN antibody
    • Nuclear orphan receptor LXR beta antibody
    • Nuclear receptor NER antibody
    • Nuclear receptor subfamily 1 group H member 2 antibody
    • OR-1 antibody
    • Oxysterols receptor LXR-beta antibody
    • RIP15 antibody
    • Steroid hormone nuclear receptor NER antibody
    • Ubiquitously-expressed nuclear receptor antibody
    • UNR antibody
    see all

Anti-LXR beta antibody images

  • Anti-LXR beta antibody (ab76983) at 5 µg/ml + IMR-32 cell lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 51 kDa
    Observed band size : 51 kDa
  • Immunofluorescence analysis of LXR beta expression in HeLa cells using 10 µg/ml of ab76983
  • Detection limit for ab76983 in Sandwich ELISA (using recombinant protein) is approximately 0.03 ng/ml as a capture antibody.
  • Overlay histogram showing HeLa cells stained with ab76983 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76983, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

References for Anti-LXR beta antibody (ab76983)

This product has been referenced in:
  • McDonald G  et al. Normalizing glycosphingolipids restores function in CD4+ T cells from lupus patients. J Clin Invest 124:712-24 (2014). Human . Read more (PubMed: 24463447) »
  • Kruse MS  et al. Alterations of LXRa and LXRß expression in the hypothalamus of glucose-intolerant rats. J Endocrinol 215:51-8 (2012). IHC ; Rat . Read more (PubMed: 22836489) »

See all 2 Publications for this product

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Efectivamente el péptido usado para producir ab3585 es ab5021, y por tanto puede usarse en los ensayos de bloqueo del mismo. Sin embargo no hay ninguna publicación disponible acerca de este ensayo de bloqu...

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We have several antibodies against LXR beta (ab24361, ab56237, ab58089, ab58092, ab76983, ab106473) . The tested application/species/unit size can be found on the on-line product d...

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Application Western blot
Sample Human Cell lysate - whole cell (THP-1 cells)
Loading amount 35 µg
Specification THP-1 cells
Gel Running Conditions Reduced Denaturing (12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Dec 07 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"