Overview

  • Product nameAnti-Ly49F antibody [HBF-719] (FITC)
    See all Ly49F primary antibodies
  • Description
    Mouse monoclonal [HBF-719] to Ly49F (FITC)
  • ConjugationFITC. Ex: 493nm, Em: 528nm
  • SpecificityThis antibody is specific for the B6 allele of the Ly49F receptor.
  • Tested applicationsSuitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    CHO cells transfected with the B6 allele of Ly49F.

  • General notes


    This antibody stains ~ 11% of NK1.1+CD3- splenocytes, and 3.5% (2.5 month old) to 10.0% (11 month old) of CD8+ spleen cells from C57BL/6 mice.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityProtein G purified
  • Primary antibody notesThis antibody stains ~ 11% of NK1.1+CD3- splenocytes, and 3.5% (2.5 month old) to 10.0% (11 month old) of CD8+ spleen cells from C57BL/6 mice.
  • ClonalityMonoclonal
  • Clone numberHBF-719
  • IsotypeIgG1
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab25018 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µg for 106 cells. ab106163-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • RelevanceLy49F is a member of the lectin like homodimeric Ly49 family of cell surface receptors. Mouse Ly49 receptors exhibit allelic specificity for MHC class I Ia molecules and are thought to serve to prevent natural killer (NK) cells from attacking normal cells, while allowing them to attack infected or transformed cells in which class I molecules have been down regulated. These inhibitory receptors are also expressed on subpopulations of mouse CD8+ T cells.
  • Cellular localizationType II membrane protein.
  • Database links
  • Alternative names
    • Killer cell lectin like receptor 6 antibody
    • Klra6 antibody
    • Ly49F antigen antibody
    • Lymphocyte antigen 49F antibody
    • T cell surface glycoprotein Ly 49F antibody
    see all

References for Anti-Ly49F antibody [HBF-719] (FITC) (ab25018)

This product has been referenced in:

See 1 Publication for this product

Product Wall

Thank you for contacting us. I have attached FACS data for the current lot of clone HBF-719, lot GR57605-1, referred to as lot "H7803-XJ23" on the histogram/dot blots. The histograms to the left show the Ly49F-positive sub-population, about 9% ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"