Overview

  • Product name
  • Description
    Rabbit polyclonal to Ly6c
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 50 to the C-terminus of Mouse Ly6c.

    (Peptide available as ab96115.)

  • Positive control
    • This antibody gave a positive signal in Mouse thymus tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab77766 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 11 kDa (predicted molecular weight: 14 kDa).

Target

  • Relevance
    Ly6C is a monocyte/macrophage and endothelial cell differentiation antigen regulated by interferon gamma, and may play a role in the development and maturation of lymphocytes. It is a member of the Ly6 multigene family of type V glycophosphatidylinositol anchored cell surface proteins. It is expressed on bone marrow cells, monocytes/macrophages, neutrophils, endothelial cells, and T cell subsets. Mice with the Ly6.2 allotype (e.g., AKR, C57BL, C57BR, C57L, DBA/2, PL, SJL, SWR, 129) have subsets of CD4+Ly6C+ and CD8+Ly6C+ cells, while Ly6.1 strains (e.g., A, BALB/c, CBA, C3H/He, DBA/1, NZB) have only CD8+Ly6C+ lymphocytes.
  • Cellular localization
    Cell membrane; Lipid-anchor, GPI-anchor.
  • Database links
  • Alternative names
    • Ly 6c antibody
    • Ly6c protein antibody
    • Ly6c1 antibody
    • Ly6c2 antibody
    • Lymphocyte antigen 6 complex antibody
    • Lymphocyte antigen 6 complex locus C antibody
    • Lymphocyte antigen 6C antibody
    • Lymphocyte antigen 6C1 antibody
    • Lymphocyte antigen 6C2 antibody
    • Lymphocyte antigen Ly 6C antibody
    see all

Images

  • Anti-Ly6c antibody (ab77766) at 1 µg/ml + Thymus (Mouse) Tissue Lysate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 14 kDa
    Observed band size: 11 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 19 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 20 minutes


    We hypothesize that the 11 kDa represents the mature form of Mouse Lymphocyte antigen 6C precursor. Ly6c has a 26 amino acid signal peptide which, when cleaved, gives an expected molecular weight of 11 kDa.

References

ab77766 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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