Anti-Ly6c antibody [ER-MP 20] (FITC) (ab15686)


  • Product nameAnti-Ly6c antibody [ER-MP 20] (FITC)
    See all Ly6c primary antibodies
  • Description
    Rat monoclonal [ER-MP 20] to Ly6c (FITC)
  • ConjugationFITC. Ex: 493nm, Em: 528nm
  • Specificityab15686 recognizes murine Ly6C, a 14kD differentiation antigen, which is expressed on macrophage/dendritic cell precursors in midstage development (late CFUM, monoblasts and immature monocytes), granulocytes, and on a wide range of endothelial cells and subpopulations of B and T lymphocytes. It is ideally suited for the detection of monocytes in bone marrow samples by FACS. It also identifies activated macrophages in inflammatory tissues where the simultaneous use of the murine pan-macrophage marker is recommended.
  • Tested applicationsSuitable for: ICC/IF, Flow Cyt, ICCmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Tissue, cells or virus corresponding to Mouse Ly6c. Also; SwissProt ID P0CW03.
    Database link: P0CW02

  • EpitopeSpleen cells from immunised rats were fused with cells of the Y3Ag1.2.3 myeloma cell line.
  • General notes

    ab15686 is useful for the detection of macrophage precursor cells in the mid-stage development stage (late CFU-M, monoblasts and monocytes). It is highly suitable for the detection of monocytes in bone marrow samples by FACS.



Our Abpromise guarantee covers the use of ab15686 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
Flow Cyt 1/10 - 1/20. ab18446-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
ICC 1/200.


  • RelevanceLy6C is a monocyte/macrophage and endothelial cell differentiation antigen regulated by interferon gamma, and may play a role in the development and maturation of lymphocytes. It is a member of the Ly6 multigene family of type V glycophosphatidylinositol anchored cell surface proteins. It is expressed on bone marrow cells, monocytes/macrophages, neutrophils, endothelial cells, and T cell subsets. Mice with the Ly6.2 allotype (e.g., AKR, C57BL, C57BR, C57L, DBA/2, PL, SJL, SWR, 129) have subsets of CD4+Ly6C+ and CD8+Ly6C+ cells, while Ly6.1 strains (e.g., A, BALB/c, CBA, C3H/He, DBA/1, NZB) have only CD8+Ly6C+ lymphocytes.
  • Cellular localizationCell membrane; Lipid-anchor, GPI-anchor.
  • Database links
  • Alternative names
    • Ly 6c antibody
    • Ly6c protein antibody
    • Ly6c1 antibody
    • Ly6c2 antibody
    • Lymphocyte antigen 6 complex antibody
    • Lymphocyte antigen 6 complex locus C antibody
    • Lymphocyte antigen 6C antibody
    • Lymphocyte antigen 6C1 antibody
    • Lymphocyte antigen 6C2 antibody
    • Lymphocyte antigen Ly 6C antibody
    see all

References for Anti-Ly6c antibody [ER-MP 20] (FITC) (ab15686)

ab15686 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (MEF)
Specification MEF
Fixative none, used on live cells
Permeabilization No
Blocking step Serum as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 37°C

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Submitted Oct 03 2007