Overview

  • Product nameAnti-Ly6c antibody [ER-MP20]
    See all Ly6c primary antibodies
  • Description
    Rat monoclonal [ER-MP20] to Ly6c
  • Specificityab15627 recognizes murine Ly6C, a 14kD differentiation antigen, which is expressed on macrophage/dendritic cell precursors in midstage development (late CFUM, monoblasts and immature monocytes), granulocytes, and on a wide range of endothelial cells and subpopulations of B and T lymphocytes. It is ideally suited for the detection of monocytes in bone marrow samples by FACS. It also identifies activated macrophages in inflammatory tissues where the simultaneous use of the murine pan-macrophage marker is recommended.
  • Tested applicationsSuitable for: IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Tissue, cells or virus corresponding to Mouse Ly6c. Also; SwissProt ID P0CW03.
    Database link: P0CW02

  • EpitopeSpleen cells from immunised rats were fused with cells of the Y3Ag1.2.3 myeloma cell line.
  • Positive control
    • Monoblasts, late CFU M cells, monocytes, granulocytes. Macrophage precursor subpopulations in the bone marrow and hemopoietic islands of the lymphoid organs, and in the spleen. Endothelial cells of small vessels in various organs. Activated macrophages in inflammatory tissues.
  • General notes

    ab15627 is useful for the detection of macrophage precursor cells in the mid-stage development stage (late CFU-M, monoblasts and monocytes). It is highly suitable for the detection of monocytes in bone marrow samples by FACS. ab15627 also identifies activated macrophages in inflammatory tissues where the simultaneous use of the murine pan-macrophage marker BM8 is recommended. ab15674 also detects a wide range of endothelial cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab15627 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 0.5 µg/ml.
IHC-Fr Use at an assay dependent concentration.

Target

  • RelevanceLy6C is a monocyte/macrophage and endothelial cell differentiation antigen regulated by interferon gamma, and may play a role in the development and maturation of lymphocytes. It is a member of the Ly6 multigene family of type V glycophosphatidylinositol anchored cell surface proteins. It is expressed on bone marrow cells, monocytes/macrophages, neutrophils, endothelial cells, and T cell subsets. Mice with the Ly6.2 allotype (e.g., AKR, C57BL, C57BR, C57L, DBA/2, PL, SJL, SWR, 129) have subsets of CD4+Ly6C+ and CD8+Ly6C+ cells, while Ly6.1 strains (e.g., A, BALB/c, CBA, C3H/He, DBA/1, NZB) have only CD8+Ly6C+ lymphocytes.
  • Cellular localizationCell membrane; Lipid-anchor, GPI-anchor.
  • Database links
  • Alternative names
    • Ly 6c antibody
    • Ly6c protein antibody
    • Ly6c1 antibody
    • Ly6c2 antibody
    • Lymphocyte antigen 6 complex antibody
    • Lymphocyte antigen 6 complex locus C antibody
    • Lymphocyte antigen 6C antibody
    • Lymphocyte antigen 6C1 antibody
    • Lymphocyte antigen 6C2 antibody
    • Lymphocyte antigen Ly 6C antibody
    see all

Anti-Ly6c antibody [ER-MP20] images

  • ab15627 staining Ly6c in Mouse tumor stroma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde, permeablizied with Tween-20 and blocked with 1% BSA for 2 hours at room temperature; antigen retrieval was by heat mediation in citrate pH6. Samples were incubated with primary antibody (1/100 in 1% BSA + 1% FBS) for 16 hours. An undiluted HRP-conjugated goat anti-rat IgG polyclonal was used as the secondary antibody. A Avidin / Streptovidin amplification kit was used followed by DAB development.

    See Abreview

  • ab15627 staining Ly6c in murine lung tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
    Lungs were excised, fixed in 10% neutral-buffered formalin and embedded in paraffin for hematoxylin and eosin staining. Immunohistochemical analysis was performed with heat-induced antigen retrieval. Biotinylated secondary antibody was used with an ABC kit and DAB detection kit to reveal the positively stained cells with nuclei counterstained with hematoxylin.

    Scale bar 200 µm.

References for Anti-Ly6c antibody [ER-MP20] (ab15627)

This product has been referenced in:
  • Liu G  et al. Targeting S1P1 receptor protects against murine immunological hepatic injury through myeloid-derived suppressor cells. J Immunol 192:3068-79 (2014). Read more (PubMed: 24567529) »
  • Chakrabarty P  et al. IFN-gamma promotes complement expression and attenuates amyloid plaque deposition in amyloid beta precursor protein transgenic mice. J Immunol 184:5333-43 (2010). IHC-P ; Mouse . Read more (PubMed: 20368278) »

See all 4 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Rat Tissue sections (Brain)
Antigen retrieval step None
Permeabilization Yes - 0.2% Triton-100X
Specification Brain
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C
Fixative Paraformaldehyde
Username

Ms. Yao Guo

Verified customer

Submitted Jan 28 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Human fibromatosis)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris EDTA pH 9
Permeabilization Yes - Tween-20
Specification Human fibromatosis
Blocking step BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

Submitted Jun 30 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Mouse tumor stroma)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate pH6
Permeabilization Yes - Tween-20
Specification Mouse tumor stroma
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Jun 17 2015

The production and characterization of the ER-MP20 clone are discussed in the following reference.

Murine macrophage precursor characterization. II. Monoclonal antibodies against macrophage precursor antigens.
Eur J Immunol. 1990 Jan;20(1)...

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Thank you for your enquiry. The antigen retrieval method used with paraffin sections with product ab15627 is digestion with proteinase K. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"