Overview

  • Product nameAnti-Ly6c antibody [HK1.4] (Allophycocyanin)
    See all Ly6c primary antibodies
  • Description
    Rat monoclonal [HK1.4] to Ly6c (Allophycocyanin)
  • ConjugationAllophycocyanin. Ex: 645nm, Em: 660nm
  • Tested applicationsSuitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • Positive control
    • BALB/c splenocytes
  • General notes


    In vitro addition of clone HK1.4 monoclonal antibody can increase proliferation and stimulate cytokine release.

Properties

Applications

Our Abpromise guarantee covers the use of ab93550 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 0.125µg for 105-8 cells.

0.125µg for 105 to 108 cells in a final volume of 100 µl.





ab154435-Rat monoclonal IgG2c, is suitable for use as an isotype control with this antibody.

Target

  • RelevanceLy6C is a monocyte/macrophage and endothelial cell differentiation antigen regulated by interferon gamma, and may play a role in the development and maturation of lymphocytes. It is a member of the Ly6 multigene family of type V glycophosphatidylinositol anchored cell surface proteins. It is expressed on bone marrow cells, monocytes/macrophages, neutrophils, endothelial cells, and T cell subsets. Mice with the Ly6.2 allotype (e.g., AKR, C57BL, C57BR, C57L, DBA/2, PL, SJL, SWR, 129) have subsets of CD4+Ly6C+ and CD8+Ly6C+ cells, while Ly6.1 strains (e.g., A, BALB/c, CBA, C3H/He, DBA/1, NZB) have only CD8+Ly6C+ lymphocytes.
  • Cellular localizationCell membrane; Lipid-anchor, GPI-anchor.
  • Database links
  • Alternative names
    • Ly 6c antibody
    • Ly6c protein antibody
    • Ly6c1 antibody
    • Ly6c2 antibody
    • Lymphocyte antigen 6 complex antibody
    • Lymphocyte antigen 6 complex locus C antibody
    • Lymphocyte antigen 6C antibody
    • Lymphocyte antigen 6C1 antibody
    • Lymphocyte antigen 6C2 antibody
    • Lymphocyte antigen Ly 6C antibody
    see all

Anti-Ly6c antibody [HK1.4] (Allophycocyanin) images

  • Staining of BALB/c splenocytes with FITC anti-mouse CD8 and 0.06 µg of ab93550. Total viable cells were used for analysis.

References for Anti-Ly6c antibody [HK1.4] (Allophycocyanin) (ab93550)

This product has been referenced in:
  • Pflugh DL  et al. Ly-6 superfamily members Ly-6A/E, Ly-6C, and Ly-6I recognize two potential ligands expressed by B lymphocytes. J Immunol 169:5130-6 (2002). Read more (PubMed: 12391229) »
  • Pflugh DL  et al. Ly-6 superfamily members Ly-6A/E, Ly-6C, and Ly-6I recognize two potential ligands expressed by B lymphocytes. J Immunol 169:5130-6 (2002). Read more (PubMed: 12391229) »

See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"