The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 0.2µg for 106 cells. ab154450-Rat monoclonal IgG2c, is suitable for use as an isotype control with this antibody.
Use at an assay dependent concentration.
Use at an assay dependent concentration. In vitro activation of T lymphocytes.
RelevanceLy6C is a monocyte/macrophage and endothelial cell differentiation antigen regulated by interferon gamma, and may play a role in the development and maturation of lymphocytes. It is a member of the Ly6 multigene family of type V glycophosphatidylinositol anchored cell surface proteins. It is expressed on bone marrow cells, monocytes/macrophages, neutrophils, endothelial cells, and T cell subsets. Mice with the Ly6.2 allotype (e.g., AKR, C57BL, C57BR, C57L, DBA/2, PL, SJL, SWR, 129) have subsets of CD4+Ly6C+ and CD8+Ly6C+ cells, while Ly6.1 strains (e.g., A, BALB/c, CBA, C3H/He, DBA/1, NZB) have only CD8+Ly6C+ lymphocytes.
Flow Cytometry - Anti-Ly6c antibody [HK1.4] (Phycoerythrin) (ab25572)This image is courtesy of an anonymous Abreview
Flow cytometry analysis of murine splenocytes, staining Ly6c with ab25572.
Cells were cultured in Cells were isolated via mechanical disruption of the spleen. The sample was incubated with the primary antibody (1/100 in 2% FCS + 0.5 mM EDTA in PBS) for 30 minutes at 4°C.
Gating Strategy: lymphocytes
References for Anti-Ly6c antibody [HK1.4] (Phycoerythrin) (ab25572)
This product has been referenced in:
Olme CH et al. Live cell detection of chromosome 2 deletion and Sfpi1/PU1 loss in radiation-induced mouse acute myeloid leukaemia. Leuk Res37:1374-82 (2013).
Read more (PubMed: 23806234) »
Ocuin LM et al. Neutrophil IL-10 suppresses peritoneal inflammatory monocytes during polymicrobial sepsis. J Leukoc Biol89:423-32 (2011).
Read more (PubMed: 21106642) »