Anti-Ly6c antibody [HK1.4] (Phycoerythrin) (ab25572)


  • Product nameAnti-Ly6c antibody [HK1.4] (Phycoerythrin)
    See all Ly6c primary antibodies
  • Description
    Rat monoclonal [HK1.4] to Ly6c (Phycoerythrin)
  • ConjugationPhycoerythrin. Ex: 488nm, Em: 575nm
  • Specificityab25572 recognises Ly6c.
  • Tested applicationsSuitable for: Flow Cyt, IHC-Fr, Cellular Activationmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    The details of the immunogen for this antibody are not available.



Our Abpromise guarantee covers the use of ab25572 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 0.2µg for 106 cells. ab154450-Rat monoclonal IgG2c, is suitable for use as an isotype control with this antibody.
IHC-Fr Use at an assay dependent concentration.
Cellular Activation Use at an assay dependent concentration. In vitro activation of T lymphocytes.


  • RelevanceLy6C is a monocyte/macrophage and endothelial cell differentiation antigen regulated by interferon gamma, and may play a role in the development and maturation of lymphocytes. It is a member of the Ly6 multigene family of type V glycophosphatidylinositol anchored cell surface proteins. It is expressed on bone marrow cells, monocytes/macrophages, neutrophils, endothelial cells, and T cell subsets. Mice with the Ly6.2 allotype (e.g., AKR, C57BL, C57BR, C57L, DBA/2, PL, SJL, SWR, 129) have subsets of CD4+Ly6C+ and CD8+Ly6C+ cells, while Ly6.1 strains (e.g., A, BALB/c, CBA, C3H/He, DBA/1, NZB) have only CD8+Ly6C+ lymphocytes.
  • Cellular localizationCell membrane; Lipid-anchor, GPI-anchor.
  • Database links
  • Alternative names
    • Ly 6c antibody
    • Ly6c protein antibody
    • Ly6c1 antibody
    • Ly6c2 antibody
    • Lymphocyte antigen 6 complex antibody
    • Lymphocyte antigen 6 complex locus C antibody
    • Lymphocyte antigen 6C antibody
    • Lymphocyte antigen 6C1 antibody
    • Lymphocyte antigen 6C2 antibody
    • Lymphocyte antigen Ly 6C antibody
    see all

Anti-Ly6c antibody [HK1.4] (Phycoerythrin) images

  • Flow cytometry analysis of murine splenocytes, staining Ly6c with ab25572.

    Cells were cultured in Cells were isolated via mechanical disruption of the spleen. The sample was incubated with the primary antibody (1/100 in 2% FCS + 0.5 mM EDTA in PBS) for 30 minutes at 4°C.

    Gating Strategy: lymphocytes

References for Anti-Ly6c antibody [HK1.4] (Phycoerythrin) (ab25572)

This product has been referenced in:
  • Olme CH  et al. Live cell detection of chromosome 2 deletion and Sfpi1/PU1 loss in radiation-induced mouse acute myeloid leukaemia. Leuk Res 37:1374-82 (2013). Flow Cyt ; Mouse . Read more (PubMed: 23806234) »
  • Ocuin LM  et al. Neutrophil IL-10 suppresses peritoneal inflammatory monocytes during polymicrobial sepsis. J Leukoc Biol 89:423-32 (2011). Flow Cyt ; Mouse . Read more (PubMed: 21106642) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Mouse Cell (splenocytes)
Specification splenocytes
Preparation Cell harvesting/tissue preparation method: mechanical disruption of spleen
Sample buffer: 2% FCS, 0.5 mM EDTA in PBS
Fixation Paraformaldehyde
Permeabilization No
Gating Strategy lymphocytes

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Submitted Sep 19 2012