Anti-Ly6g antibody [RB6-8C5] (Allophycocyanin) (ab25273)

Overview

  • Product name
    Anti-Ly6g antibody [RB6-8C5] (Allophycocyanin)
    See all Ly6g primary antibodies
  • Description
    Rat monoclonal [RB6-8C5] to Ly6g (Allophycocyanin)
  • Conjugation
    Allophycocyanin. Ex: 645nm, Em: 660nm
  • Tested applications
    Suitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    The details of the immunogen for this antibody are not available.

Properties

Applications

Our Abpromise guarantee covers the use of ab25273 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

Target

  • Relevance
    Ly6G is a GPI-anchored protein, that is also known as the myeloid differentiation antigen Gr1. The antigen is transiently expressed on monocytes in the bone marrow. The level of antigen expression in the bone marrow directly correlates with granulocyte differentiation and maturation. Ly6G is expressed predominantly on neutrophils, also in a subset of eosinophils, differentiating pre-monocytes, and plasmacytoid dendritic cells.
  • Cellular localization
    Attached to the membrane by a GPI-anchor.
  • Database links
  • Alternative names
    • Gr-1 antibody
    • Gr1 antibody
    • Ly-6G.1 antibody
    • Ly6G antibody
    • Lymphocyte antigen 6 complex locus G antibody
    • Lymphocyte antigen 6G antibody
    see all

References for Anti-Ly6g antibody [RB6-8C5] (Allophycocyanin) (ab25273)

ab25273 has not yet been referenced specifically in any publications.

Product Wall

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Thank you for submitting an Abreview for ab25273. As you may have noticed, your review has now been published on our website.
Since you obtained poor results using the antibody in an untested species (dog), we would like to follow up on this to see...

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Abcam has not validated the combination of species/application used in this Abreview.
Application
Flow Cytometry
Sample
Dog Cell (from whole blood)
Specification
from whole blood
Preparation
Cell harvesting/tissue preparation method: after red blood cell lysis
Sample buffer: PBS/BSA 1%
Fixation
Paraformaldehyde
Permeabilization
No
Gating Strategy
alive cells
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Verified customer

Submitted Nov 06 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Cynomolgus Monkey Cell (cells from whole blood)
Specification
cells from whole blood
Preparation
Cell harvesting/tissue preparation method: after red blood cell lysis
Sample buffer: PBS/BSA 1%
Fixation
Paraformaldehyde
Permeabilization
No
Gating Strategy
alive neutrophils
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Verified customer

Submitted Nov 02 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Spleen from immune mouse)
Specification
Spleen from immune mouse
Fixative
Acetone
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Verified customer

Submitted Apr 16 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application
Flow Cytometry
Sample
Rat Cell (Splenocytes)
Specification
Splenocytes
Preparation
Cell harvesting/tissue preparation method: spleen was mashed
Sample buffer: PBS/BSA 1%/Azide 0.01%
Permeabilization
No
Gating Strategy
alive macrophages
Username

Abcam user community

Verified customer

Submitted Apr 16 2012

Thank you for contacting Abcam. I am sorry to hear you have experienced difficulty obtaining satisfactory results from this antibody.

I would be happy to arrange a Free of Charge Replacement of this product for you. We do have one product ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Mouse Cell (cells from mouse blood)
Specification
cells from mouse blood
Preparation
Cell harvesting/tissue preparation method: after red blood cell lysis
Sample buffer: PBS/BSA 1%
Permeabilization
No
Gating Strategy
on neutrophils
Username

Abcam user community

Verified customer

Submitted Dec 20 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Human Cell (cell from buffy coat)
Specification
cell from buffy coat
Preparation
Cell harvesting/tissue preparation method: none
Sample buffer: PBS/BSA 1%
Permeabilization
No
Gating Strategy
on granulocytes
Username

Abcam user community

Verified customer

Submitted Dec 20 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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