Anti-Ly6g antibody [RB6-8C5] (Cy5 ®) (ab24985)

Overview

  • Product name
    Anti-Ly6g antibody [RB6-8C5] (Cy5 ®)
    See all Ly6g primary antibodies
  • Description
    Rat monoclonal [RB6-8C5] to Ly6g (Cy5 ®)
  • Conjugation
    Cy5 ®. Ex: 650nm, Em: 667nm
  • Tested applications
    Suitable for: Flow Cyt, IHC-Fr, IP, WBmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • General notes
    This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited.

Properties

Applications

Our Abpromise guarantee covers the use of ab24985 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
IHC-Fr
IP
WB
  • Application notes
    Flow Cyt: Use 0.2µg for 106 cells.
    IHC-Fr: Use at an assay dependent dilution.
    IP: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution. Predicted molecular weight: 21 kDa.
    Complement-dependent cytotoxicity assays: Use at an assay dependent dilution.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      Ly6G is a GPI-anchored protein, that is also known as the myeloid differentiation antigen Gr1. The antigen is transiently expressed on monocytes in the bone marrow. The level of antigen expression in the bone marrow directly correlates with granulocyte differentiation and maturation. Ly6G is expressed predominantly on neutrophils, also in a subset of eosinophils, differentiating pre-monocytes, and plasmacytoid dendritic cells.
    • Cellular localization
      Attached to the membrane by a GPI-anchor.
    • Database links
    • Alternative names
      • Gr-1 antibody
      • Gr1 antibody
      • Ly-6G.1 antibody
      • Ly6G antibody
      • Lymphocyte antigen 6 complex locus G antibody
      • Lymphocyte antigen 6G antibody
      see all

    References

    ab24985 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate
    Sample
    Rat Tissue sections (Brain)
    Specification
    Brain
    Permeabilization
    Yes - 0.4% Triton X-100
    Fixative
    Formaldehyde
    Username

    Dr. S Gajavelli

    Verified customer

    Submitted Aug 20 2014

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Sodium Citrate
    Sample
    Mouse Tissue sections (lung)
    Specification
    lung
    Permeabilization
    Yes - Triton X-100
    Fixative
    Formalin/PFA-fixed paraffin-embedded sections
    Username

    Abcam user community

    Verified customer

    Submitted May 02 2014

    Thank you very much for your interest in Abcam and our products..

    To our knowledge,the following antibodies you listed have not been tested in IHC-P. Tested applications and species are listed on the individual datasheets. These are updated ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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