Anti-Ly6g antibody [RB6-8C5] (FITC) (ab25024)

Overview

  • Product nameAnti-Ly6g antibody [RB6-8C5] (FITC)
    See all Ly6g primary antibodies
  • Description
    Rat monoclonal [RB6-8C5] to Ly6g (FITC)
  • ConjugationFITC. Ex: 493nm, Em: 528nm
  • Specificityab25024 recognises mouse Mouse Ly-6G.
  • Tested applicationsSuitable for: Flow Cyt, IP, IHC-Fr, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Monkey
  • Immunogen

    The details of the immunogen for this antibody are not available.

Properties

Applications

Our Abpromise guarantee covers the use of ab25024 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µg for 106 cells. ab136125-Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
IP Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 21-25 kDa.
In vitro complement-dependent cytotoxicity assays: Use at an assay dependent dilution.
In vivo depletion of Ly-6G+ cells: Use at an assay dependent dilution.

Target

  • RelevanceLy6G is a GPI-anchored protein, that is also known as the myeloid differentiation antigen Gr1. The antigen is transiently expressed on monocytes in the bone marrow. The level of antigen expression in the bone marrow directly correlates with granulocyte differentiation and maturation. Ly6G is expressed predominantly on neutrophils, also in a subset of eosinophils, differentiating pre-monocytes, and plasmacytoid dendritic cells.
  • Cellular localizationAttached to the membrane by a GPI-anchor.
  • Database links
  • Alternative names
    • Gr-1 antibody
    • Gr1 antibody
    • Ly-6G.1 antibody
    • Ly6G antibody
    • Lymphocyte antigen 6 complex locus G antibody
    • Lymphocyte antigen 6G antibody
    see all

Anti-Ly6g antibody [RB6-8C5] (FITC) images

  • BALB/c bone marrow cells were stained with either rat IgG2b-FITC (as an isotype control) or rat anti-mouse Ly-6G-FITC (ab25024) at a concentration of 1ug/106 cells.  Large cells were then gated and analyzed on a flow cytometer.

  • ab25024 at a 1/100 dilution detecting Ly6g in LPS activated monocytes by Flow Cytometry.
    Gating strategy was for alive cells.

    See Abreview

  • ab25024 at a 1/100 dilution detecting Ly6g (Gr1) in murine blood cells by Flow Cytometry.
    Gating strategy was for monocytes.

    See Abreview

  • ab25024 staining Ly6g in Cynomolgus Monkey whole blood by Flow Cytometry. Red blood cells were lysed in PBS + 1% BSA and 0.01% sodium azide and fixed in paraformaldehyde. The sample was incubated with the primary antibody (1/10 in PBS + 1% BSA and 0.01% sodium azide) for 1 hour at 4°C.
    Gating Strategy: Live Monocytes.

    See Abreview

  • ab25024 detecting Ly6g in rat spleenocytes by Flow Cytometry. Spleen tissue was homogenized and red blood cells were lysed in PBS + 1% BSA and 0.01% sodium azide. The sample was incubated with the primary antibody (1/100 in PBS + 1% BSA and 0.01% sodium azide) for 1 hour at 4°C.
    Gating Strategy: Live macrophages.

    See Abreview

  • Flow Cytometry analysis of BALB/c mouse bone marrow cells labeling Mouse Ly-6G/Ly-6C with ab25024 at 1 μg/106 cells. A Rat Anti-Mouse CD19-PE was used as the secondary antibody.

References for Anti-Ly6g antibody [RB6-8C5] (FITC) (ab25024)

This product has been referenced in:
  • Chen D  et al. Protease-activated receptor 1 activation is necessary for monocyte chemoattractant protein 1-dependent leukocyte recruitment in vivo. J Exp Med 205:1739-46 (2008). Flow Cyt ; Mouse . Read more (PubMed: 18606855) »

See 1 Publication for this product

Product Wall

Having reviewed this case, I would like to offer some suggestions to help optimize the results from ab25024. I would also appreciate if you can confirm some further details: 1) I see in your details that you are using 0.75ul (0.375ug) of antibody fo...

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Thank you for submitting an Abreview for ab25024. As you may have noticed, your review has now been published on our website.
Since you obtained poor results using the antibody in an untested species (dog), we would like to follow up on this to see...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Flow Cytometry
Sample Dog Cell (from whole blood)
Specification from whole blood
Preparation Cell harvesting/tissue preparation method: after red blood cell lysis
Sample buffer: PBS/BSA 1%
Fixation Paraformaldehyde
Permeabilization No
Gating Strategy alive cells
Username

Abcam user community

Verified customer

Submitted Nov 06 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Rat Cell (Splenocytes)
Specification Splenocytes
Preparation Cell harvesting/tissue preparation method: spleen was mashed and red blood cells lysed
Sample buffer: PBS/BSA 1%/Azide 0.01%
Permeabilization No
Gating Strategy alive macrophages
Username

Abcam user community

Verified customer

Submitted Apr 19 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Spleen from immune mouse)
Specification Spleen from immune mouse
Fixative Acetone
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Apr 16 2012

Application Flow Cytometry
Sample Cynomolgus Monkey Cell (Whole blood)
Specification Whole blood
Preparation Cell harvesting/tissue preparation method: After red blood cell lysis
Sample buffer: PBS/BSA 1%/Azide 0.01%
Fixation Paraformaldehyde
Permeabilization No
Gating Strategy alive monocytes
Username

Abcam user community

Verified customer

Submitted Apr 16 2012

Thank you for your patience.


I have now had the Rat IgG1 (FITC) isotype control added to our catalog, ab128477.


I hope this information is helpful. Please do not hesitate to contact us if you have any additional questions.

Thank you for contacting Abcam regarding ab25024.


I am working to have the appropriate isotype control added to our catalog for you to purchase. I will provide the ordering details as soon as they are available.


I hope t...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (LPS activated monocytes)
Specification LPS activated monocytes
Permeabilization No
Gating Strategy alive cells
Username

Abcam user community

Verified customer

Submitted Dec 23 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Mouse Cell (cells from blood)
Specification cells from blood
Permeabilization No
Gating Strategy monocytes
Username

Abcam user community

Verified customer

Submitted Dec 23 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"