Anti-Ly6g antibody [RB6-8C5] (PE/Cy7 ®) (ab25514)
- Datasheet
- Specific References (11)
- Protocols
Overview
- Product nameAnti-Ly6g antibody [RB6-8C5] (PE/Cy7 ®)
See all Ly6g primary antibodies - DescriptionRat monoclonal [RB6-8C5] to Ly6g (PE/Cy7 ®)
- ConjugationPE/Cy7 ®. Ex: 496nm, Em: 774nm
- Tested applicationsSuitable for: Flow Cyt, IHC-Fr, IP, WBmore details
- Species reactivityReacts with: Mouse
- Immunogen
The details of the immunogen for this antibody are not available.
- Positive control
- Flow Cyt: C57Bl/6 bone marrow cells.
- General notesThis product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited.
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Store at +4°C. Avoid freeze / thaw cycle. Store In the Dark.
- Storage bufferpH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 0.1% Gelatin, 0.88% Sodium chloride, 0.12% Monobasic dihydrogen sodium phosphate -
Concentration information loading... - PurityAffinity purified
- ClonalityMonoclonal
- Clone numberRB6-8C5
- IsotypeIgG2b
- Light chain typekappa
- Research areas
Associated products
- Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab25514 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use at an assay dependent concentration. ab154461 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
|
| IHC-Fr | Use at an assay dependent concentration. | |
| IP | Use at an assay dependent concentration. In vitro complement-dependent cytotoxicity assays: Use at an assay dependent dilution. In vivo depletion of Ly-6G+ cells: Use at an assay dependent dilution. |
|
| WB | Use at an assay dependent concentration. Predicted molecular weight: 14 kDa. |
Target
- RelevanceLy6G is a GPI-anchored protein, that is also known as the myeloid differentiation antigen Gr1. The antigen is transiently expressed on monocytes in the bone marrow. The level of antigen expression in the bone marrow directly correlates with granulocyte differentiation and maturation. Ly6G is expressed predominantly on neutrophils, also in a subset of eosinophils, differentiating pre-monocytes, and plasmacytoid dendritic cells.
- Cellular localizationAttached to the membrane by a GPI-anchor.
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Database links
- Entrez Gene: 546644 Mouse
- SwissProt: P35461 Mouse
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Alternative names
- Gr-1 antibody
- Gr1 antibody
- Ly-6G.1 antibody
see all
Anti-Ly6g antibody [RB6-8C5] (PE/Cy7 ®) images
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![Flow Cytometry - Anti-Ly6g antibody [RB6-8C5] (PE/Cy7 ®) (ab25514)](http://a.abcam.com/ps/products/25/ab25514/Images/ab25514-277202-ab25514fc.jpg)
Flow Cytometry - Anti-Ly6g antibody [RB6-8C5] (PE/Cy7 ®) (ab25514)C57Bl/6 bone marrow cells stained with 0.25µg ab25514 (solid line) or 0.25µg PE-Cy7-conjugated rat IgG2b isotype control (dashed line).
References for Anti-Ly6g antibody [RB6-8C5] (PE/Cy7 ®) (ab25514)
This product has been referenced in:
- Tumpey TM et al. Neutrophil-mediated suppression of virus replication after herpes simplex virus type 1 infection of the murine cornea. J Virol 70:898-904 (1996). Read more (PubMed: 8551629) »
- Lagasse E & Weissman IL Flow cytometric identification of murine neutrophils and monocytes. J Immunol Methods 197:139-50 (1996). Read more (PubMed: 8890901) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"