Overview

  • Product name
    Anti-Ly6g antibody [RB6-8C5] (PE/Cy7 ®)
    See all Ly6g primary antibodies
  • Description
    Rat monoclonal [RB6-8C5] to Ly6g (PE/Cy7 ®)
  • Host species
    Rat
  • Conjugation
    PE/Cy7 ®. Ex: 496nm, Em: 774nm
  • Tested applications
    Suitable for: Flow Cyt, IHC-Fr, IP, WBmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • Positive control
    • Flow Cyt: C57Bl/6 bone marrow cells.
  • General notes
    This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited.

    Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab25514 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

ab154461 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration.

In vitro complement-dependent cytotoxicity assays: Use at an assay dependent dilution.

In vivo depletion of Ly-6G+ cells: Use at an assay dependent dilution.
WB Use at an assay dependent concentration. Predicted molecular weight: 14 kDa.

Target

  • Relevance
    Ly6G is a GPI-anchored protein, that is also known as the myeloid differentiation antigen Gr1. The antigen is transiently expressed on monocytes in the bone marrow. The level of antigen expression in the bone marrow directly correlates with granulocyte differentiation and maturation. Ly6G is expressed predominantly on neutrophils, also in a subset of eosinophils, differentiating pre-monocytes, and plasmacytoid dendritic cells.
  • Cellular localization
    Attached to the membrane by a GPI-anchor.
  • Database links
    • Alternative names
      • Gr-1 antibody
      • Gr1 antibody
      • Ly-6G.1 antibody
      • Ly6G antibody
      • Lymphocyte antigen 6 complex locus G antibody
      • Lymphocyte antigen 6G antibody
      see all

    Images

    • Flow Cytometry - Anti-Ly6g antibody [RB6-8C5] (PE/Cy7 ®) (ab25514)
      Flow Cytometry - Anti-Ly6g antibody [RB6-8C5] (PE/Cy7 ®) (ab25514)

      C57Bl/6 bone marrow cells stained with 0.25µg ab25514 (solid line) or 0.25µg PE-Cy7-conjugated rat IgG2b isotype control (dashed line).

    References

    This product has been referenced in:
    • Tumpey TM  et al. Neutrophil-mediated suppression of virus replication after herpes simplex virus type 1 infection of the murine cornea. J Virol 70:898-904 (1996). Read more (PubMed: 8551629) »
    • Lagasse E & Weissman IL Flow cytometric identification of murine neutrophils and monocytes. J Immunol Methods 197:139-50 (1996). Read more (PubMed: 8890901) »

    See all 11 Publications for this product

    Publishing research using ab25514? Please let us know so that we can cite the reference in this datasheet.

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