PE/Cy7® Anti-Ly6g antibody [RB6-8C5] (ab25514)
Key features and details
- PE/Cy7® Rat monoclonal [RB6-8C5] to Ly6g
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: PE/Cy7®. Ex: 496nm, Em: 774nm
- Isotype: IgG2b
Related conjugates and formulations
Overview
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Product name
PE/Cy7® Anti-Ly6g antibody [RB6-8C5]
See all Ly6g primary antibodies -
Description
PE/Cy7® Rat monoclonal [RB6-8C5] to Ly6g -
Host species
Rat -
Conjugation
PE/Cy7®. Ex: 496nm, Em: 774nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Tissue, cells or virus corresponding to Ly6g. Mouse granulocytes
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Positive control
- Flow Cyt: C57Bl/6 bone marrow cells.
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General notes
This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5, 268, 486 and related patents. Cy® and CyDye® are trademarks of Cytiva.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 0.1% Gelatin, 0.88% Sodium chloride, 0.12% Monobasic dihydrogen sodium phosphate -
Concentration information loading...
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Purity
Affinity purified -
Purification notes
Purified from TCS -
Clonality
Monoclonal -
Clone number
RB6-8C5 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
- redFluor™ 710 Anti-Ly6g antibody [RB6-8C5] (ab242065)
- PE Anti-Ly6g antibody [RB6-8C5] (ab24884)
- FITC Anti-Ly6g antibody [RB6-8C5] (ab25024)
- APC Anti-Ly6g antibody [RB6-8C5] (ab25273)
- violetFluor™ 450 Anti-Ly6g antibody [RB6-8C5] (ab253070)
- Anti-Ly6g + Ly6c antibody [RB6-8C5] (ab25377)
- Biotin Anti-Ly6g antibody [RB6-8C5] (ab25680)
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab25514 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt |
Use at an assay dependent concentration.
ab154461 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
Notes |
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Flow Cyt
Use at an assay dependent concentration. ab154461 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
Target
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Relevance
Ly6G is a GPI-anchored protein, that is also known as the myeloid differentiation antigen Gr1. The antigen is transiently expressed on monocytes in the bone marrow. The level of antigen expression in the bone marrow directly correlates with granulocyte differentiation and maturation. Ly6G is expressed predominantly on neutrophils, also in a subset of eosinophils, differentiating pre-monocytes, and plasmacytoid dendritic cells. -
Cellular localization
Attached to the membrane by a GPI-anchor. -
Database links
- Entrez Gene: 546644 Mouse
- SwissProt: P35461 Mouse
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Alternative names
- Gr-1 antibody
- Gr1 antibody
- Ly-6G.1 antibody
see all
Images
Protocols
Datasheets and documents
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Datasheet download
References (13)
ab25514 has been referenced in 13 publications.
- Baccelli I et al. Mubritinib Targets the Electron Transport Chain Complex I and Reveals the Landscape of OXPHOS Dependency in Acute Myeloid Leukemia. Cancer Cell 36:84-99.e8 (2019). PubMed: 31287994
- Ho TC et al. The Psoriasis Therapeutic Potential of a Novel Short Laminin Peptide C16. Int J Mol Sci 20:N/A (2019). PubMed: 31252620
- Tumpey TM et al. Neutrophil-mediated suppression of virus replication after herpes simplex virus type 1 infection of the murine cornea. J Virol 70:898-904 (1996). PubMed: 8551629
- Lagasse E & Weissman IL Flow cytometric identification of murine neutrophils and monocytes. J Immunol Methods 197:139-50 (1996). PubMed: 8890901
- Rakhmilevich AL Neutrophils are essential for resolution of primary and secondary infection with Listeria monocytogenes. J Leukoc Biol 57:827-31 (1995). PubMed: 7790763