Overview

  • Product nameAnti-Ly6g antibody [RB6-8C5] (PE/Cy7 ®)
    See all Ly6g primary antibodies
  • Description
    Rat monoclonal [RB6-8C5] to Ly6g (PE/Cy7 ®)
  • ConjugationPE/Cy7 ®. Ex: 496nm, Em: 774nm
  • Tested applicationsSuitable for: Flow Cyt, IHC-Fr, IP, WBmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • General notesThis product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited.

Properties

Applications

Our Abpromise guarantee covers the use of ab25514 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration. ab154461-Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
In vitro complement-dependent cytotoxicity assays:Use at an assay dependent dilution.
In vivo depletion of Ly-6G+ cells:Use at an assay dependent dilution.
WB Use at an assay dependent concentration. Predicted molecular weight: 14 kDa.

Target

  • RelevanceLy6G is a GPI-anchored protein, that is also known as the myeloid differentiation antigen Gr1. The antigen is transiently expressed on monocytes in the bone marrow. The level of antigen expression in the bone marrow directly correlates with granulocyte differentiation and maturation. Ly6G is expressed predominantly on neutrophils, also in a subset of eosinophils, differentiating pre-monocytes, and plasmacytoid dendritic cells.
  • Cellular localizationAttached to the membrane by a GPI-anchor.
  • Database links
  • Alternative names
    • Gr-1 antibody
    • Gr1 antibody
    • Ly-6G.1 antibody
    • Ly6G antibody
    • Lymphocyte antigen 6 complex locus G antibody
    • Lymphocyte antigen 6G antibody
    see all

Anti-Ly6g antibody [RB6-8C5] (PE/Cy7 ®) images

  • Flow Cytometry analysis of BALB/c mouse bone marrow cells labeling Ly-6G-PE/CY7 with ab25514 at 0.01 μg/106 cells. A Rat Anti-Mouse CD19-PE was used as the secondary antibody.

References for Anti-Ly6g antibody [RB6-8C5] (PE/Cy7 ®) (ab25514)

This product has been referenced in:
  • Tumpey TM  et al. Neutrophil-mediated suppression of virus replication after herpes simplex virus type 1 infection of the murine cornea. J Virol 70:898-904 (1996). Read more (PubMed: 8551629) »
  • Lagasse E & Weissman IL Flow cytometric identification of murine neutrophils and monocytes. J Immunol Methods 197:139-50 (1996). Read more (PubMed: 8890901) »

See all 11 Publications for this product

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"