Overview

  • Product name
  • Description
    Rabbit polyclonal to LY6G6F
  • Tested applications
    Suitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Dog
  • Immunogen

    A 14-22 amino acid region within synthetic peptide: SPPTLHGDEH LSWFCSPAAG SFTTLVAQVQ VGRPAPDPGK PGRESRLRLL, corresponding to N terminal amino acids 37-86 of Human LY6G6F

  • Positive control
    • HepG2 cell lysate and human kidney tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab62597 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/312500.

Target

  • Function
    May play a role in the downstream signal transduction pathways involving GRB2 and GRB7.
  • Sequence similarities
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Post-translational
    modifications
    N-glycosylated.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • C6orf21 antibody
    • Chromosome 6 open reading frame 21 antibody
    • G6F antibody
    • G6f protein [Precursor] antibody
    • HCG43720, isoform CRA_c antibody
    • LY66F_HUMAN antibody
    • LY6G6D antibody
    • Ly6g6f antibody
    • Lymphocyte antigen 6 complex locus G6F antibody
    • Lymphocyte antigen 6 complex locus protein G6f antibody
    • Lymphocyte antigen 6 complex, locus G6D antibody
    • NG32 antibody
    see all

Images

  • Anti-LY6G6F antibody (ab62597) at 1.25 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 32 kDa
    Observed band size : 32 kDa
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling LY6G6F with ab62597 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of the renal tubule. Magnification: 400X.

References

ab62597 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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