The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 0.03µg for 105-8 cells. ab18427-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
Acts as an endocytic receptor to direct captured antigens from the extracellular space to a specialized antigen-processing compartment (By similarity). Causes reduced proliferation of B-lymphocytes.
Expressed in spleen, thymus, colon and peripheral blood lymphocytes. Detected in myeloid and B lymphoid cell lines. Isoform 2 and isoform 3 are expressed in malignant Hodgkin lymphoma cells called Hodgkin and Reed-Sternberg (HRS) cells.
Staining of normal Human peripheral blood cells (left) or mature dendritic cells (right) with Mouse IgG2b ? Isotype Control FITC (open histogram) or 0.015 µg of ab95586
(filled histogram). Total viable cells were used for analysis.