Overview

  • Product name
    Anti-LY75 antibody [NLDC-145] (FITC)
    See all LY75 primary antibodies
  • Description
    Rat monoclonal [NLDC-145] to LY75 (FITC)
  • Conjugation
    FITC. Ex: 493nm, Em: 528nm
  • Specificity
    Reacts with non lymphoid dendritic cells: interdigitating cells (IDC), veiled cells, Langerhans cells and thymic epithelial cells; recognises gp200 localized on the cell surface and intracellularly.
  • Tested applications
    Suitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Mouse lymph node tissue.

  • Epitope
    The antigen detected by NLDC-145 is an integral membrane glycoprotein with an apparent mass of 205 kDa, also known as DEC-205.
  • Positive control
    • GM-CSF and IL4 stimulated bone marrow cells.
  • General notes
    The antigen is expressed at high levels by dendritic cells and thymic epithelial cells.


    Monoclonal antibody NLDC-145 identifies Ia positive interdigitating cells, veiled cells and Langerhans cells of the skin and their in vitro counterparts.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: PBS, 10mg/ml BSA, pH 7.2
  • Concentration information loading...
  • Purity
    Protein A purified
  • Primary antibody notes
    Monoclonal antibody NLDC-145 identifies Ia positive interdigitating cells, veiled cells and Langerhans cells of the skin and their in vitro counterparts.
  • Clonality
    Monoclonal
  • Clone number
    NLDC-145
  • Isotype
    IgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab51817 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration. ab18446-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Acts as an endocytic receptor to direct captured antigens from the extracellular space to a specialized antigen-processing compartment (By similarity). Causes reduced proliferation of B-lymphocytes.
  • Tissue specificity
    Expressed in spleen, thymus, colon and peripheral blood lymphocytes. Detected in myeloid and B lymphoid cell lines. Isoform 2 and isoform 3 are expressed in malignant Hodgkin lymphoma cells called Hodgkin and Reed-Sternberg (HRS) cells.
  • Sequence similarities
    Contains 10 C-type lectin domains.
    Contains 1 fibronectin type-II domain.
    Contains 1 ricin B-type lectin domain.
  • Post-translational
    modifications
    N-glycosylated.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • C-type lectin domain family 13 member B antibody
    • CD 205 antibody
    • CD205 antibody
    • CD205 antigen antibody
    • CLEC 13B antibody
    • CLEC13B antibody
    • DEC 205 antibody
    • DEC-205 antibody
    • DEC205 antibody
    • gp200 antibody
    • gp200 MR6 antibody
    • gp200-MR6 antibody
    • LY 75 antibody
    • Ly-75 antibody
    • LY75 antibody
    • LY75_HUMAN antibody
    • Lymphocyte antigen 75 antibody
    • Renal Cell Carcinoma (gp200) antibody
    see all

References

This product has been referenced in:
  • Swiggard WJ  et al. DEC-205, a 205-kDa protein abundant on mouse dendritic cells and thymic epithelium that is detected by the monoclonal antibody NLDC-145: purification, characterization, and N-terminal amino acid sequence. Cell Immunol 165:302-11 (1995). Read more (PubMed: 7553896) »
  • Breel M  et al. Dendritic cells of the mouse recognized by two monoclonal antibodies. Eur J Immunol 17:1555-9 (1987). Read more (PubMed: 3678361) »

See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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