The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration. Predicted molecular weight: 198 kDa.
Use a concentration of 2 - 4 µg/ml.
Use for 30 min at RT. Staining of formalin-fixed tissues requires digestion with Protease XXV at 1mg/ml PBS for 10 minutes at 37°C.
FunctionActs as an endocytic receptor to direct captured antigens from the extracellular space to a specialized antigen-processing compartment (By similarity). Causes reduced proliferation of B-lymphocytes.
Tissue specificityExpressed in spleen, thymus, colon and peripheral blood lymphocytes. Detected in myeloid and B lymphoid cell lines. Isoform 2 and isoform 3 are expressed in malignant Hodgkin lymphoma cells called Hodgkin and Reed-Sternberg (HRS) cells.
References for Anti-LY75 antibody [PN-15] (Biotin) (ab79458)
This product has been referenced in:
Yoshida SO & Imam A Monoclonal antibody to a proximal nephrogenic renal antigen: immunohistochemical analysis of formalin-fixed, paraffin-embedded human renal cell carcinomas. Cancer Res49:1802-9 (1989).
Read more (PubMed: 2466563) »