Overview

  • Product name
    Anti-Ly76 antibody [TER-119] (PE/Cy5®)
    See all Ly76 primary antibodies
  • Description
    Rat monoclonal [TER-119] to Ly76 (PE/Cy5®)
  • Conjugation
    PE/Cy5®. Ex: 496nm, Em: 670nm
  • Tested applications
    Suitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Day-14 fetal liver cells from a C57BL/6 mouse.

  • Positive control
    • C57Bl/6 bone marrow cells; Mouse splenocytes

Properties

Applications

Our Abpromise guarantee covers the use of ab95823 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 0.125-0.25µg for 105 cells. Cell number should be determined empirically but can range from 105 to 108 cells/test (based on a test volume of 100 µl).

Target

  • Relevance
    Ly76 is a glycophorin A-associated protein. It is an erythroid-specific antigen expressed on early proerythroblasts to mature erythrocytes, but not BFU-E or CFU-E.
  • Cellular localization
    Plasma membrane
  • Database links
  • Alternative names
    • Ly 76 antibody
    • Lymphocyte antigen 76 antibody
    • TER 119 antibody
    • TER119 antibody

Images

  • Flow cytometry staining of C57Bl/6 bone marrow cells with staining buffer (autofluorescence) (open histogram) or 0.125 µg of ab95823 (filled histogram). Total cells were used for analysis.

References

This product has been referenced in:
  • Kina T  et al. The monoclonal antibody TER-119 recognizes a molecule associated with glycophorin A and specifically marks the late stages of murine erythroid lineage. Br J Haematol 109:280-7 (2000). Read more (PubMed: 10848813) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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