The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesFlow Cyt: Use at a concentration of 0.25 µg/test.
A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 105 to 108 cells/test.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceLy76 is a glycophorin A-associated protein. It is an erythroid-specific antigen expressed on early proerythroblasts to mature erythrocytes, but not BFU-E or CFU-E.
flow cytometric analysis showing staining of BALB/c bone marrow cells with 0.125 µg of PE Rat IgG2b Isotype Control (open histogram) or 0.125 µg of ab93586 (colored histogram).
References for Anti-Ly76 antibody [TER-119] (Phycoerythrin) (ab93586)
This product has been referenced in:
Kina T et al. The monoclonal antibody TER-119 recognizes a molecule associated with glycophorin A and specifically marks the late stages of murine erythroid lineage. Br J Haematol109:280-7 (2000).
Read more (PubMed: 10848813) »
Vannucchi AM et al. Identification and characterization of a bipotent (erythroid and megakaryocytic) cell precursor from the spleen of phenylhydrazine-treated mice. Blood95:2559-68 (2000).
Read more (PubMed: 10753835) »