Anti-Ly76 antibody [TER-119] (Phycoerythrin) (ab93586)

Overview

  • Product nameAnti-Ly76 antibody [TER-119] (Phycoerythrin)
    See all Ly76 primary antibodies
  • Description
    Rat monoclonal [TER-119] to Ly76 (Phycoerythrin)
  • ConjugationPhycoerythrin. Ex: 488nm, Em: 575nm
  • Tested applicationsSuitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Tissue/ cell preparation - Mouse fetal liver cells

  • Positive control
    • BALB/c bone marrow cells

Properties

Applications

Our Abpromise guarantee covers the use of ab93586 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

Target

  • RelevanceLy76 is a glycophorin A-associated protein. It is an erythroid-specific antigen expressed on early proerythroblasts to mature erythrocytes, but not BFU-E or CFU-E.
  • Cellular localizationPlasma membrane
  • Database links
  • Alternative names
    • Ly 76 antibody
    • Lymphocyte antigen 76 antibody
    • TER 119 antibody
    • TER119 antibody

Anti-Ly76 antibody [TER-119] (Phycoerythrin) images

  • Flow cytometry of C57Bl/6 bone marrow cells labeling Ly76 with ab93586 at 0.5µg (solid line) or Rat IgG2b isotype control at 0.5µg (dashed line).

References for Anti-Ly76 antibody [TER-119] (Phycoerythrin) (ab93586)

This product has been referenced in:
  • Kina T  et al. The monoclonal antibody TER-119 recognizes a molecule associated with glycophorin A and specifically marks the late stages of murine erythroid lineage. Br J Haematol 109:280-7 (2000). Read more (PubMed: 10848813) »
  • Vannucchi AM  et al. Identification and characterization of a bipotent (erythroid and megakaryocytic) cell precursor from the spleen of phenylhydrazine-treated mice. Blood 95:2559-68 (2000). Read more (PubMed: 10753835) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Mouse Cell (Erythrocytes)
Specification Erythrocytes
Preparation Cell harvesting/tissue preparation method: Isolation of purified erythrocytes from whole blood using percoll gradient
Sample buffer: PBS with 5mM EDTA
Fixation Unfixed
Permeabilization No
Gating Strategy live cell population of purified erythrocytes gated on the basis of FSC/SSC. P2 gating for positive cell population was set so that <1% of the negative cell population was PE positive.
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Submitted Mar 25 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"