Recombinant
RabMAb

Anti-LYAR antibody [EPR14352] (ab181261)

Overview

  • Product name
    Anti-LYAR antibody [EPR14352]
    See all LYAR primary antibodies
  • Description
    Rabbit monoclonal [EPR14352] to LYAR
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human LYAR aa 150-350. The exact sequence is proprietary.
    Database link: Q9NX58

  • Positive control
    • HeLa, A431, Ramos and Jurkat cell lysates; HeLa cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR14352
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab181261 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.
WB 1/10000 - 1/50000. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa).

Target

  • Sequence similarities
    Contains 2 C2HC-type zinc fingers.
  • Cellular localization
    Nucleus > nucleolus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cell growth regulating nucleolar protein antibody
    • Cell growth-regulating nucleolar protein antibody
    • Likely ortholog of mouse Ly1 antibody reactive clone antibody
    • Ly1 antibody reactive antibody
    • Ly1 antibody reactive homolog (mouse) antibody
    • Ly1 antibody reactive homolog antibody
    • LYAR antibody
    • LYAR_HUMAN antibody
    • ZC2HC2 antibody
    • ZLYAR antibody
    see all

Images

  • All lanes : Anti-LYAR antibody [EPR14352] (ab181261) at 1/20000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : Ramos cell lysate
    Lane 3 : Jurkat cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L) HRP at 1/1000 dilution

    Predicted band size: 44 kDa

  • Anti-LYAR antibody [EPR14352] (ab181261) at 1/10000 dilution + A431 cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L) HRP at 1/1000 dilution

    Predicted band size: 44 kDa

  • Immunofluorescent analysis of HeLa cells (-20°C acetone-fixed) labeling LYAR with ab181261 at 1/100 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody at 1/200 dilution and counter-stained with DAPI (blue).

References

ab181261 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab181261.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up