• Product nameAnti-LYK5 antibody
    See all LYK5 primary antibodies
  • Description
    Rabbit polyclonal to LYK5
  • SpecificityThis antibody detects endogenous levels of total LYK5 protein.
  • Tested applicationsSuitable for: IHC-P, WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    A synthetic peptide derived from an internal region of human LYK5.

  • Positive control
    • Extracts from HepG2 cells.



Our Abpromise guarantee covers the use of ab64799 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/500 - 1/1000. Detects a band of approximately 46 kDa (predicted molecular weight: 48 kDa).
ELISA 1/10000.


  • RelevanceMembers of the STE-20 like kinase family are known to stimulate MAPK pathways by directly activating MAPKKK. LYK5 is a novel pseudokinase member of this family consisting of a STE-20 like kinase domain but lacks several residues that are required for its catalytic activity. It specifically binds LKB1 and plays a key role in regulating the tumor suppressor activities of LKB1. It functions as an upstream activator of LKB1 and also directs the sub-cellular localization of LKB1 by anchoring it in the cytoplasm. LYK5-LKB1 interaction results in phosphorylation of LYK5 and enhanced autophosphorylation of LKB1.
  • Cellular localizationCytoplasmic and Nuclear
  • Database links
  • Alternative names
    • Protein kinase LYK5 antibody
    • Serologically defined breast cancer antigen NY BR 96 antibody
    • STE20 related adapter protein antibody
    • STRAD alpha antibody
    • STRAD antibody
    see all

Anti-LYK5 antibody images

  • All lanes : Anti-LYK5 antibody (ab64799) at 1/500 dilution

    Lane 1 : Extracts from HepG2 cells
    Lane 2 : Extracts from HepG2 cells, plus peptide

    Lysates/proteins at 5 µg per lane.

    Predicted band size : 48 kDa
    Observed band size : 46 kDa (why is the actual band size different from the predicted?)
  • IHC image of ab64799 staining in human liver cancer formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab64799, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-LYK5 antibody (ab64799)

ab64799 has not yet been referenced specifically in any publications.

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