Overview

  • Product nameAnti-LYK5 antibody
    See all LYK5 primary antibodies
  • Description
    Rabbit polyclonal to LYK5
  • Tested applicationsSuitable for: ICC/IF, ELISA, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide from the N-terminal region of human LYK5, conjugated to KLH.

  • Positive control
    • Mouse brain tissue lysate. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: HepG2

Properties

Applications

Our Abpromise guarantee covers the use of ab71476 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
ELISA 1/1000.
WB 1/100 - 1/500. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).

Target

  • RelevanceMembers of the STE-20 like kinase family are known to stimulate MAPK pathways by directly activating MAPKKK. LYK5 is a novel pseudokinase member of this family consisting of a STE-20 like kinase domain but lacks several residues that are required for its catalytic activity. It specifically binds LKB1 and plays a key role in regulating the tumor suppressor activities of LKB1. It functions as an upstream activator of LKB1 and also directs the sub-cellular localization of LKB1 by anchoring it in the cytoplasm. LYK5-LKB1 interaction results in phosphorylation of LYK5 and enhanced autophosphorylation of LKB1.
  • Cellular localizationCytoplasmic and Nuclear
  • Database links
  • Alternative names
    • Protein kinase LYK5 antibody
    • Serologically defined breast cancer antigen NY BR 96 antibody
    • STE20 related adapter protein antibody
    • STRAD alpha antibody
    • STRAD antibody
    see all

Anti-LYK5 antibody images

  • ab71476 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab71476 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-LYK5 antibody (ab71476) at 1/100 dilution + mouse brain tissue lysate at 12.5 µg

    Predicted band size : 48 kDa
    Observed band size : 48 kDa
    Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.

References for Anti-LYK5 antibody (ab71476)

This product has been referenced in:
  • Asada N & Sanada K LKB1-mediated spatial control of GSK3beta and adenomatous polyposis coli contributes to centrosomal forward movement and neuronal migration in the developing neocortex. J Neurosci 30:8852-65 (2010). WB ; Mouse . Read more (PubMed: 20592207) »

See 1 Publication for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Sample Rat Tissue sections (brain)
Permeabilization Yes - triton
Specification brain
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
Fixative Paraformaldehyde
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Submitted Nov 06 2015

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