Human Predicted to work with:
Mouse, Rat, Cow, Cynomolgus Monkey, Orangutan
Recombinant fragment: LLEKLNGTVP CLLDTSTIPA EELTMSPSRS VANSGLSDSL TTSTPRPSNG DSPSHPYHRT FSPHFHHFVE QCLQRNPDAR YPCWPGPGLR ESRGCS, corresponding to amino acids 251-346 of human LYK5 with a 26 kDa tag (NP_699166).
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesELISA: Use at an assay dependent dilution.
WB: Use at a concentration of 1 - 5 µg/ml. Predicted molecular weight for isoform 2: 39 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceMembers of the STE-20 like kinase family are known to stimulate MAPK pathways by directly activating MAPKKK. LYK5 is a novel pseudokinase member of this family consisting of a STE-20 like kinase domain but lacks several residues that are required for its catalytic activity. It specifically binds LKB1 and plays a key role in regulating the tumor suppressor activities of LKB1. It functions as an upstream activator of LKB1 and also directs the sub-cellular localization of LKB1 by anchoring it in the cytoplasm. LYK5-LKB1 interaction results in phosphorylation of LYK5 and enhanced autophosphorylation of LKB1.