Overview

  • Product nameAnti-LYK5 antibody
    See all LYK5 primary antibodies
  • Description
    Mouse monoclonal to LYK5
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Cynomolgus Monkey, Orangutan
  • Immunogen

    Recombinant fragment: LLEKLNGTVP CLLDTSTIPA EELTMSPSRS VANSGLSDSL TTSTPRPSNG DSPSHPYHRT FSPHFHHFVE QCLQRNPDAR YPCWPGPGLR ESRGCS, corresponding to amino acids 251-346 of human LYK5 with a 26 kDa tag (NP_699166).

  • Positive control
    • HeLa nuclear cell lysate; Recombinant tagged fragment of human LYK5 (the immunogen).

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab77016 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notesELISA: Use at an assay dependent dilution.
    WB: Use at a concentration of 1 - 5 µg/ml. Predicted molecular weight for isoform 2: 39 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceMembers of the STE-20 like kinase family are known to stimulate MAPK pathways by directly activating MAPKKK. LYK5 is a novel pseudokinase member of this family consisting of a STE-20 like kinase domain but lacks several residues that are required for its catalytic activity. It specifically binds LKB1 and plays a key role in regulating the tumor suppressor activities of LKB1. It functions as an upstream activator of LKB1 and also directs the sub-cellular localization of LKB1 by anchoring it in the cytoplasm. LYK5-LKB1 interaction results in phosphorylation of LYK5 and enhanced autophosphorylation of LKB1.
    • Cellular localizationCytoplasmic and Nuclear
    • Database links
    • Alternative names
      • Protein kinase LYK5 antibody
      • Serologically defined breast cancer antigen NY BR 96 antibody
      • STE20 related adapter protein antibody
      • STRAD alpha antibody
      • STRAD antibody
      see all

    Anti-LYK5 antibody images

    • Anti-LYK5 antibody (ab77016) at 5 µg/ml + recombinant fragment of human LYK5 at 0.2 µg

      Secondary
      Goat anti-Mouse IgG (H&L)-HRP at 1/5000 dilution

      Predicted band size : 39 kDa
      Observed band size : 37 kDa (why is the actual band size different from the predicted?)
    • Anti-LYK5 antibody (ab77016) at 1/500 dilution + HeLa cell lysate at 25 µg

      Secondary
      Goat anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

      Predicted band size : 39 kDa
      Observed band size : 36 kDa (why is the actual band size different from the predicted?)

    References for Anti-LYK5 antibody (ab77016)

    ab77016 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"