Recombinant
RabMAb

Anti-LYK5 antibody [EPR15603] (ab192879)

Overview

  • Product name
    Anti-LYK5 antibody [EPR15603]
    See all LYK5 primary antibodies
  • Description
    Rabbit monoclonal [EPR15603] to LYK5
  • Tested applications
    Suitable for: Flow Cyt, WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human LYK5 aa 250-400. The exact sequence is proprietary.
    Database link: Q7RTN6

  • Positive control
    • 293, HepG2, HeLa and Human fetal brain lysates; Human kidney and rat cardiac muscle tissues; A549 cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab192879 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

Purified format.

WB 1/1000 - 1/10000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF 1/400.

Target

  • Relevance
    Members of the STE-20 like kinase family are known to stimulate MAPK pathways by directly activating MAPKKK. LYK5 is a novel pseudokinase member of this family consisting of a STE-20 like kinase domain but lacks several residues that are required for its catalytic activity. It specifically binds LKB1 and plays a key role in regulating the tumor suppressor activities of LKB1. It functions as an upstream activator of LKB1 and also directs the sub-cellular localization of LKB1 by anchoring it in the cytoplasm. LYK5-LKB1 interaction results in phosphorylation of LYK5 and enhanced autophosphorylation of LKB1.
  • Cellular localization
    Cytoplasmic and Nuclear
  • Database links
  • Alternative names
    • Protein kinase LYK5 antibody
    • Serologically defined breast cancer antigen NY BR 96 antibody
    • STE20 related adapter protein antibody
    • STRAD alpha antibody
    • STRAD antibody
    see all

Images

  • Flow cytometry analysis of HeLa (human cervix adenocarcinoma) cells labelling LYK5 antibody (red) with purified ab192879 at a dilution of 1/200. Goat anti rabbit IgG (Alexa Fluor® 488) was used as the secondary antibody at 1/2000. Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Isotype control antibody was Rabbit monoclonal IgG (black). The blue line shows cells without incubation with primary antibody and secondary antibody.

  • All lanes : Anti-LYK5 antibody [EPR15603] (ab192879) at 1/5000 dilution

    Lane 1 : 293 cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : Human fetal brain lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size : 48 kDa
  • Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling LYK5 with ab192879 at a 1/100 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG as a secondary antibody. Counter stained with Hematoxylin. Negative control also shown.

  • Immunohistochemical analysis of paraffin-embedded rat cardiac muscle tissue labeling LYK5 with ab192879 at a 1/100 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG as a secondary antibody. Counter stained with Hematoxylin. Negative control also shown.

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% triton X-100 permeabilized A549 cells labeling LYK5 with ab192879 at a 1/400 dilution, followed by Goat anti rabbit IgG(Alexa Fluor® 488) secondary antibody (ab150077) at 1/400 dilution. Nuclear counter stain: Dapi (blue).

References

ab192879 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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