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Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Our Abpromise guarantee covers the use of ab192879 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration.
|WB||1/1000 - 1/10000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
Flow cytometry analysis of HeLa (human cervix adenocarcinoma) cells labelling LYK5 antibody (red) with purified ab192879 at a dilution of 1/200. Goat anti rabbit IgG (Alexa Fluor® 488) was used as the secondary antibody at 1/2000. Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Isotype control antibody was Rabbit monoclonal IgG (black). The blue line shows cells without incubation with primary antibody and secondary antibody.
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling LYK5 with ab192879 at a 1/100 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG as a secondary antibody. Counter stained with Hematoxylin. Negative control also shown.
Immunohistochemical analysis of paraffin-embedded rat cardiac muscle tissue labeling LYK5 with ab192879 at a 1/100 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG as a secondary antibody. Counter stained with Hematoxylin. Negative control also shown.
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% triton X-100 permeabilized A549 cells labeling LYK5 with ab192879 at a 1/400 dilution, followed by Goat anti rabbit IgG(Alexa Fluor® 488) secondary antibody (ab150077) at 1/400 dilution. Nuclear counter stain: Dapi (blue).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"