Anti-LAG-3 antibody [11E3] (ab40465)
Key features and details
- Mouse monoclonal [11E3] to LAG-3
- Suitable for: ELISA, IP, IHC-Fr, ICC/IF, Flow Cyt, Blocking, IHC-P, WB
- Reacts with: Human, Monkey
- Isotype: IgG1
Overview
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Product name
Anti-LAG-3 antibody [11E3]
See all LAG-3 primary antibodies -
Description
Mouse monoclonal [11E3] to LAG-3 -
Host species
Mouse -
Specificity
Recognizes the first N-terminal D1 domain of LAG-3.
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Tested applications
Suitable for: ELISA, IP, IHC-Fr, ICC/IF, Flow Cyt, Blocking, IHC-P, WBmore details -
Species reactivity
Reacts with: Human, Monkey -
Immunogen
Recombinant full length protein corresponding to Human LAG-3. (soluble form)
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: PBS, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
Purified from concentrated hybridoma tissue culture supernatant by Protein A Sepharose CL-4B Flow Fast Column. Purity >95% (SDS-PAGE). -
Clonality
Monoclonal -
Clone number
11E3 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab40465 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA |
Use at an assay dependent concentration.
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IP |
Use at an assay dependent concentration.
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IHC-Fr |
Use at an assay dependent concentration.
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ICC/IF |
Use at an assay dependent concentration.
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Flow Cyt |
Use 1µg for 106 cells.
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Blocking |
Use at an assay dependent concentration.
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IHC-P | (2) |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration.
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Notes |
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ELISA
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
Flow Cyt
Use 1µg for 106 cells. |
Blocking
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. |
Target
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Function
Involved in lymphocyte activation. Binds to HLA class-II antigens. -
Tissue specificity
On cell surface of activated NK and T-lymphocytes. -
Sequence similarities
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 3902 Human
- Omim: 153337 Human
- SwissProt: P18627 Human
- Unigene: 409523 Human
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Alternative names
- CD223 antibody
- CD223 antigen antibody
- FDC protein antibody
see all
Images
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Overlay histogram showing Jurkat cells stained with ab40465 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab40465, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
Datasheets and documents
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SDS download
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Datasheet download
References (8)
ab40465 has been referenced in 8 publications.
- Tcvetkov N et al. Immune checkpoints bone marrow expression as the predictor of clinical outcome in myelodysplastic syndrome. Leuk Res Rep 14:100215 (2020). PubMed: 32695574
- Ma C et al. Ectopic expression of LAG-3 in non-small-cell lung cancer cells and its clinical significance. J Clin Lab Anal 34:e23244 (2020). PubMed: 32077528
- Zhu P et al. MiRNA505/NET1 Axis Acts as a CD8+ T-TIL Regulator in Non-Small Cell Lung Cancer. Onco Targets Ther 13:9785-9795 (2020). PubMed: 33061457
- Lee SJ et al. LAG3 in Solid Tumors as a Potential Novel Immunotherapy Target. J Immunother 42:279-283 (2019). PubMed: 31219974
- Lee WJ et al. Expression of lymphocyte-activating gene 3 and T-cell immunoreceptor with immunoglobulin and ITIM domains in cutaneous melanoma and their correlation with programmed cell death 1 expression in tumor-infiltrating lymphocytes. J Am Acad Dermatol N/A:N/A (2019). PubMed: 30880064
- Wang W et al. Characterization of LAG-3, CTLA-4, and CD8+ TIL density and their joint influence on the prognosis of patients with esophageal squamous cell carcinoma. Ann Transl Med 7:776 (2019). PubMed: 32042792
- Takada K et al. Use of the tumor-infiltrating CD8 to FOXP3 lymphocyte ratio in predicting treatment responses to combination therapy with pertuzumab, trastuzumab, and docetaxel for advanced HER2-positive breast cancer. J Transl Med 16:86 (2018). IHC-P ; Human . PubMed: 29615076
- Huard B et al. Characterization of the major histocompatibility complex class II binding site on LAG-3 protein. Proc Natl Acad Sci U S A 94:5744-9 (1997). PubMed: 9159144