Anti-Lymphocyte Activation Gene 3 antibody [11E3] (ab40465)

Overview

  • Product nameAnti-Lymphocyte Activation Gene 3 antibody [11E3]
    See all Lymphocyte Activation Gene 3 primary antibodies
  • Description
    Mouse monoclonal [11E3] to Lymphocyte Activation Gene 3
  • SpecificityRecognizes the first N-terminal D1 domain of Lymphocyte Activation Gene 3.
  • Tested applicationsSuitable for: Flow Cyt, IHC-P, IP, WB, ELISA, ICC/IF, IHC-Fr, Blockingmore details
  • Species reactivity
    Reacts with: Human, Monkey
  • Immunogen

    Recombinant human Lymphocyte Activation Gene 3 (soluble form).

Properties

Applications

Our Abpromise guarantee covers the use of ab40465 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µg for 106 cells.
IHC-P Use at an assay dependent concentration.
IP Use a concentration of 10 µg/ml.
WB Use a concentration of 5 µg/ml. Predicted molecular weight: 58 kDa.
ELISA Use a concentration of 5 µg/ml.
ICC/IF 1/100.
IHC-Fr 1/150.
Blocking Use at an assay dependent concentration.

Target

  • FunctionInvolved in lymphocyte activation. Binds to HLA class-II antigens.
  • Tissue specificityOn cell surface of activated NK and T-lymphocytes.
  • Sequence similaritiesContains 3 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD223 antibody
    • CD223 antigen antibody
    • FDC protein antibody
    • LAG-3 antibody
    • Lag3 antibody
    • LAG3_HUMAN antibody
    • Lymphocyte activation gene 3 antibody
    • Lymphocyte activation gene 3 protein antibody
    • Protein FDC antibody
    see all

Anti-Lymphocyte Activation Gene 3 antibody [11E3] images

  • ab40465 staining Lymphocyte Activation Gene 3 in Human submandibular gland (disease) tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with blocking buffer for 5 minutes at 24°C; antigen retrieval was by heat mediation in a sodium citrate buffer, pH 6.0. Samples were incubated with primary antibody (1/100 in PBS) for 2 hours at 25°C. An undiluted Biotin-conjugated anti-mouse IgG monoclonal was used as the secondary antibody.

    See Abreview

  • Overlay histogram showing Jurkat cells stained with ab40465 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab40465, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

References for Anti-Lymphocyte Activation Gene 3 antibody [11E3] (ab40465)

This product has been referenced in:
  • Huard B  et al. Characterization of the major histocompatibility complex class II binding site on LAG-3 protein. Proc Natl Acad Sci U S A 94:5744-9 (1997). Read more (PubMed: 9159144) »

See 1 Publication for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step DAKO Blocking buffer as blocking agent for 5 minute(s) · Concentration: 0% · Temperature: 24°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Sodium Citrate Buffer (pH6.0)
Sample Human Tissue sections (submandibular gland (disease))
Specification submandibular gland (disease)
Permeabilization No
Fixative Formaldehyde
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Verified customer

Submitted Dec 23 2013

Thank you very much calling Abcam earlier today. Below is the information about our testing discount program that I talked about.

Asab40465 has not been tested in Flow Cytometry. Therefore, I can offer a discount off a future purchase if you b...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"