Anti-Lymphocyte Activation Gene 3 antibody [11E3] (ab40465)


  • Product nameAnti-Lymphocyte Activation Gene 3 antibody [11E3]
    See all Lymphocyte Activation Gene 3 primary antibodies
  • Description
    Mouse monoclonal [11E3] to Lymphocyte Activation Gene 3
  • SpecificityRecognizes the first N-terminal D1 domain of Lymphocyte Activation Gene 3.
  • Tested applicationsSuitable for: Flow Cyt, IHC-P, IP, WB, ELISA, ICC/IF, IHC-Fr, Blockingmore details
  • Species reactivity
    Reacts with: Human, Monkey
  • Immunogen

    Recombinant human Lymphocyte Activation Gene 3 (soluble form).



Our Abpromise guarantee covers the use of ab40465 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µg for 106 cells.
IHC-P Use at an assay dependent concentration.
IP Use a concentration of 10 µg/ml.
WB Use a concentration of 5 µg/ml. Predicted molecular weight: 58 kDa.
ELISA Use a concentration of 5 µg/ml.
ICC/IF 1/100.
IHC-Fr 1/150.
Blocking Use at an assay dependent concentration.


  • FunctionInvolved in lymphocyte activation. Binds to HLA class-II antigens.
  • Tissue specificityOn cell surface of activated NK and T-lymphocytes.
  • Sequence similaritiesContains 3 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD223 antibody
    • CD223 antigen antibody
    • FDC protein antibody
    • LAG-3 antibody
    • Lag3 antibody
    • LAG3_HUMAN antibody
    • Lymphocyte activating 3 antibody
    • Lymphocyte activation gene 3 antibody
    • Lymphocyte activation gene 3 protein antibody
    • Protein FDC antibody
    see all

Anti-Lymphocyte Activation Gene 3 antibody [11E3] images

  • ab40465 staining Lymphocyte Activation Gene 3 in Human submandibular gland (disease) tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with blocking buffer for 5 minutes at 24°C; antigen retrieval was by heat mediation in a sodium citrate buffer, pH 6.0. Samples were incubated with primary antibody (1/100 in PBS) for 2 hours at 25°C. An undiluted Biotin-conjugated anti-mouse IgG monoclonal was used as the secondary antibody.

    See Abreview

  • Overlay histogram showing Jurkat cells stained with ab40465 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab40465, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

References for Anti-Lymphocyte Activation Gene 3 antibody [11E3] (ab40465)

This product has been referenced in:
  • Huard B  et al. Characterization of the major histocompatibility complex class II binding site on LAG-3 protein. Proc Natl Acad Sci U S A 94:5744-9 (1997). Read more (PubMed: 9159144) »

See 1 Publication for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (FFPE HDLM2 cells pellet)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate pH 6
Permeabilization No
Specification FFPE HDLM2 cells pellet
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 1.5% · Temperature: 22°C
Fixative Paraformaldehyde

Zohar Gavish

Verified customer

Submitted Dec 12 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step DAKO Blocking buffer as blocking agent for 5 minute(s) · Concentration: 0% · Temperature: 24°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Sodium Citrate Buffer (pH6.0)
Sample Human Tissue sections (submandibular gland (disease))
Specification submandibular gland (disease)
Permeabilization No
Fixative Formaldehyde

Abcam user community

Verified customer

Submitted Dec 23 2013

Thank you very much calling Abcam earlier today. Below is the information about our testing discount program that I talked about.

Asab40465 has not been tested in Flow Cytometry. Therefore, I can offer a discount off a future purchase if you b...

Read More