Anti-Lymphocyte Activation Gene 3 antibody [17B4] (ab40466)

Overview

  • Product name
    Anti-Lymphocyte Activation Gene 3 antibody [17B4]
    See all Lymphocyte Activation Gene 3 primary antibodies
  • Description
    Mouse monoclonal [17B4] to Lymphocyte Activation Gene 3
  • Tested applications
    Suitable for: IHC-Fr, IP, WB, ICC/IF, Inhibition Assaymore details
  • Species reactivity
    Reacts with: Human, Rhesus monkey
  • Immunogen

    Synthetic peptide:

    G PPAAAPGHPL APGPHPAAPS SWGPRPRRY

    , corresponding to amino acids 70-99 of Human Lymphocyte Activation Gene 3

  • Positive control
    • (Human) large B-cell lymphoma.

Properties

Applications

Our Abpromise guarantee covers the use of ab40466 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/150.
IP Use a concentration of 10 µg/ml.
WB Use a concentration of 5 µg/ml. Predicted molecular weight: 58 kDa.
ICC/IF 1/100.
Inhibition Assay Use a concentration of 10 µg/ml.

Target

  • Function
    Involved in lymphocyte activation. Binds to HLA class-II antigens.
  • Tissue specificity
    On cell surface of activated NK and T-lymphocytes.
  • Sequence similarities
    Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD223 antibody
    • CD223 antigen antibody
    • FDC protein antibody
    • LAG-3 antibody
    • Lag3 antibody
    • LAG3_HUMAN antibody
    • Lymphocyte activating 3 antibody
    • Lymphocyte activation gene 3 antibody
    • Lymphocyte activation gene 3 protein antibody
    • Protein FDC antibody
    see all

Anti-Lymphocyte Activation Gene 3 antibody [17B4] images

  • This image shows a diffuse large B cell lymphoma (human) stained with ab40466 at a 1/150 dilution.
  • ab40466 staining Lymphocyte Activation Gene 3 (red) in Rhesus monkey lung tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with Triton and blocked with Dako serum free protein block at 24°C. Samples were incubated with primary antibody (1/100) for 1 hour at 24°C. Dako permanent red was used as the secondary antibody. Nuclear staining is in green

    See Abreview

References for Anti-Lymphocyte Activation Gene 3 antibody [17B4] (ab40466)

This product has been referenced in:
  • Fusconi M  et al. Is montgomery tracheal safe-T-tube clinical failure induced by biofilm? Otolaryngol Head Neck Surg 149:269-76 (2013). Human . Read more (PubMed: 23649498) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Blocking step
(agent) for 40 minute(s) · Concentration: 100% · Temperature: 24°C
Sample
Rhesus monkey Tissue sections (Lung)
Specification
Lung
Permeabilization
Yes - Triton
Fixative
Paraformaldehyde
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Verified customer

Submitted Sep 17 2013

Western Blotting is described in the literature. Methods for stimulating shLAG-3 production are described in the attached paper. Please refer to Figure 5 for data as well.

I hope this information is helpful. Please let me know if you need any...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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