Our Abpromise guarantee covers the use of ab40466 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use a concentration of 10 µg/ml.|
|WB||Use a concentration of 5 µg/ml. Predicted molecular weight: 58 kDa.|
|Inhibition Assay||Use a concentration of 10 µg/ml.|
ab40466 staining Lymphocyte Activation Gene 3 (red) in Rhesus monkey lung tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with Triton and blocked with Dako serum free protein block at 24°C. Samples were incubated with primary antibody (1/100) for 1 hour at 24°C. Dako permanent red was used as the secondary antibody. Nuclear staining is in green