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Rabbit polyclonal to Lymphotoxin Beta
ab64835 detects endogenous levels of total Lymphotoxin Beta protein.
Synthetic peptide derived from C-terminal of human Lymphotoxin Beta
HepG2 cell extracts and HeLa cells.
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg 2+ and Ca 2+), 150mM Sodium chloride, pH 7.4
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration. PubMed: 22354988
1/500 - 1/1000.
1/500 - 1/1000. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).
Cytokine that binds to LTBR/TNFRSF3. May play a specific role in immune response regulation. Provides the membrane anchor for the attachment of the heterotrimeric complex to the cell surface. Isoform 2 is probably non-functional.
Spleen and thymus.
Belongs to the tumor necrosis factor family.
Information by UniProt
LT beta antibody
Western blot - Anti-Lymphotoxin Beta antibody (ab64835)
All lanes : Anti-Lymphotoxin Beta antibody (ab64835) at 1/500 dilution Lane 1 : HepG2 cell extract Lane 2 : HepG2 cell extract with immunising peptide Predicted band size: 25 kDa Observed band size: 25 kDa The amount of positive control loading for the WB is 5-30 ug of total protein. The amount of the peptide for the WB is 5-10 ug.
Immunocytochemistry/ Immunofluorescence - Anti-Lymphotoxin Beta antibody (ab64835)
ab64835 at 1/500 - 1/1000 dilution staining Lymphotoxin Beta in HeLa cells by Immunofluorescence, in the absence (-) or presence (+) of the immunising peptide.
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