Overview

  • Product nameAnti-Lyn antibody [LYN-01]
    See all Lyn primary antibodies
  • Description
    Mouse monoclonal [LYN-01] to Lyn
  • Tested applicationsSuitable for: Flow Cyt, ICC/IF, IP, WB, ICCmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment corresponding to Human Lyn aa 8-238.
    Sequence:

    GKDSLSDDGVDLK TQPVRNTERT IYVRDPTSNK QQRPVPESQL LPGQRFQTKDPEEQGDIVVA LYPYDGIHPD DLSFKKGEKM KVLEEHGEWW KAKSLLTKKE GFIPSNYVAKLNTLETEEWF FKDITRKDAE RQLLAPGNSA GAFLIRESET LKGSFSLSVR DFDPVHGDVIKHYKIRSLDN GGYYISPRIT FPCISDMIKH YQKQADGLCR RLEKACISPK PQKPWDKD

  • General notes


    Lyn is a member of the src family of non-receptor protein tyrosine kinases that is predominantly expressed in haematopoietic tissues. Like all members of the src family, lyn is thought to participate in signal transduction from cell surface receptors that lack intrinsic tyrosine kinase activity. It is associated with a number of cell surface receptors including the B cell antigen receptor and immunoglobulin E receptor (FceRI).

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 15mM Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • Purity>95% by SDS-PAGE
  • Purification notesPurified from hybridoma culture supernatant by protein A affinity chromatography.
  • Primary antibody notesLyn is a member of the src family of non-receptor protein tyrosine kinases that is predominantly expressed in haematopoietic tissues. Like all members of the src family, lyn is thought to participate in signal transduction from cell surface receptors that lack intrinsic tyrosine kinase activity. It is associated with a number of cell surface receptors including the B cell antigen receptor and immunoglobulin E receptor (FceRI).
  • ClonalityMonoclonal
  • Clone numberLYN-01
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab1890 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µg for 106 cells.
ICC/IF Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 58 kDa.
ICC Use at an assay dependent concentration.

Target

  • FunctionDown regulates expression of stem cell growth factor receptor (KIT). Acts as an effector of EpoR (erythropoietin receptor) in controlling KIT expression and may play a central role in erythroid differentiation during the switch between proliferation and maturation (By similarity). Acts as a positive regulator of cell movement while negatively regulating adhesion to stromal cells by inhibiting the ICAM-1-binding activity of beta-2 integrins. Acts as the mediator that relays suppressing signals from the chemokine receptor CXCR4 to beta-2 integrin LFA-1 in hematopoietic precursors. Involved in induction of stress-activated protein kinase (SAPK), but not ERK or p38 MAPK, in response to genotoxic agents. Induces SAPK by a MKK7- and MEKK1-dependent mechanism. The LYN -> MEKK1 -> MKK7 -> SAPK pathway is functional in the induction of apoptosis by genotoxic agents.
  • Tissue specificityWidely expressed in a variety of organs, tissues, and cell types such as epidermoid, hematopoietic, and neuronal cells. Expressed in primary neuroblastoma tumors.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
    Contains 1 protein kinase domain.
    Contains 1 SH2 domain.
    Contains 1 SH3 domain.
  • DomainThe protein kinase domain plays an important role in its localization in the cell membrane.
  • Post-translational
    modifications
    Ubiquitinated. Ubiquitination is SH3-dependent.
  • Cellular localizationCell membrane. Nucleus. Cytoplasm. Cytoplasm > perinuclear region. Golgi apparatus. Accumulates in the nucleus by inhibition of CRM1-mediated nuclear export. Nuclear accumulation is increased by inhibition of its kinase activity. The trafficking from the Golgi apparatus to the plasma membrane occurs in a kinase domain-dependent but kinase activity independent manner and is mediated by exocytic vesicular transport.
  • Information by UniProt
  • Database links
  • FormThis protein is known to be similar in amino acid sequence to HCK (P08631), LCK (P06239), FYN (P06241), YES1 (P07947), and SRC (P12931). Therefore, cross-reactivity with these homologous proteins may be observed. We would be happy to provide immunogen alignment information upon request.
  • Alternative names
    • AA407514 antibody
    • EC 2.7.10.2 antibody
    • FLJ26625 antibody
    • Hck 2 antibody
    • JTK 8 antibody
    • JTK8 antibody
    • Lck/Yes related novel protein tyrosine kinase antibody
    • LYN antibody
    • LYN proto oncogene, Src family tyrosine kinase antibody
    • LYN_HUMAN antibody
    • ONCOGENE LYN antibody
    • p53Lyn antibody
    • p56Lyn antibody
    • Tyrosine protein kinase LYN antibody
    • Tyrosine-protein kinase Lyn antibody
    • V yes 1 Yamaguchi sarcoma viral related oncogene homolog antibody
    • Yamaguchi sarcoma viral (v yes 1) related oncogene homolog antibody
    see all

Anti-Lyn antibody [LYN-01] images

  • Central Panel: ab1890 staining Lyn in human PMN cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% TritonX-100 + 2% BSA in PBS and blocked with 2% BSA for 30 minutes at 22°C. Samples were incubated with primary antibody 1/100 in blocking buffer for 4 hours at 37°C. An Alexa Fluor® 488-conjugated Goat polyclonal to mouse IgG, dilution 1/100, was used as secondary antibody.
    Left-hand panel: Nuclei counterstained with DAPI (blue).
    Right-hand panel: Overlay

    See Abreview

  • Anti-Lyn antibody [LYN-01] (ab1890) at 1/1000 dilution + whole cell lysate prepared from HEK293 cells at 50 µg

    Secondary
    HRP-conjugated anti-mouse at 1/10000 dilution

    Predicted band size : 58 kDa
    Observed band size : 56 kDa (why is the actual band size different from the predicted?)

    Image courtesy of an anonymous Abreview.

  • Anti-Lyn antibody [LYN-01] (ab1890) at 1/1000 dilution + Recombinant human Lyn protein (ab60306) at 0.01 µg

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/3000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 4 minutes
  • Overlay histogram showing Ramos cells stained with ab1890 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab1890, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Ramos cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

References for Anti-Lyn antibody [LYN-01] (ab1890)

This product has been referenced in:
  • Xue ZH  et al. Integrin alphaMbeta2 clustering triggers phosphorylation and activation of protein kinase Cdelta that regulates transcription factor Foxp1 expression in monocytes. J Immunol 184:3697-709 (2010). IP ; Human . Read more (PubMed: 20190138) »
  • Verhagen AM  et al. A kinase-dead allele of Lyn attenuates autoimmune disease normally associated with Lyn deficiency. J Immunol 182:2020-9 (2009). WB ; Mouse . Read more (PubMed: 19201855) »

See all 8 Publications for this product

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Read More
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cultured Cells (RAW 264.7)
Specification RAW 264.7
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Triton-X100 in 2% BSA for 15min
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 4°C
Username

Dr. Mahesh Shivananjappa

Verified customer

Submitted Jun 22 2012

Application Flow Cytometry
Sample Human Cell (Platelets)
Specification Platelets
Preparation Cell harvesting/tissue preparation method: PL were isolated spinning Platelet rich plasma on Histopaque
Sample buffer: PBS
Fixation Paraformaldehyde
Permeabilization Yes - 0.1% Triton-X100 in 2% BSA for 30min
Gating Strategy Platelets
Username

Dr. Mahesh Shivananjappa

Verified customer

Submitted Dec 17 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HEK-293)
Loading amount 50 µg
Specification HEK-293
Gel Running Conditions Reduced Denaturing (10% SDS-PAGE)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Oct 01 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Platelet Lysate (precleared))
Loading amount 25 µg
Specification Platelet Lysate (precleared)
Gel Running Conditions Reduced Denaturing (4-15%)
Blocking step Starting Block - Thermo as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 22°C
Username

Dr. Mahesh Shivananjappa

Verified customer

Submitted Jan 14 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (PMN)
Specification PMN
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% TritonX-100 + 2% BSA in PBS
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 22°C
Username

Dr. Mahesh Shivananjappa

Verified customer

Submitted Dec 22 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (bone marrow derived mast cells)
Loading amount 10 µg
Specification bone marrow derived mast cells
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Mr. Stefan Schattgen

Verified customer

Submitted Sep 25 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"